花生DREB类转录因子基因AhDREB3的序列及非生物胁迫下表达分析

Sequence and expression analysis of DREB transcription factor gene AhDREB3 in peanut under abiotic stress

DREB类蛋白属于AP2/ERF转录因子家族的一个亚家族,在植物非生物胁迫抗性调控中具有重要功能。为了挖掘花生逆境胁迫相关功能基因AhDREB3,从已公布的花生基因中找到干旱应答元件结合蛋白3(AhDREB3)的编码基因全序列,做编码蛋白的进化分析。根据已知序列设计引物,通过荧光定量PCR检测了该基因在低温、高盐和干旱胁迫下及对外源ABA响应和表达。荧光定量PCR结果显示,AhDREB3基因在花生的叶片和根中对低温没有响应,对高盐(叶片和根)和干旱(根)胁迫有较大响应,说明它可能参与了花生对高盐和干旱胁迫的适应性调控。此外,AhDREB3基因的表达在花生叶片和根中对外源ABA响应变化小,暗示了该基因在花生中可能通过不依赖于ABA的方式起作用。

DREB belongs to an important subfamily of AP2/ERF transcription factor families,and have important functions in regulation of plant abiotic stress resistance.To screen the potential function of stress-related gene AhDREB3 in peanut,full-length sequence of the drought-responsive element-binding protein 3(AhDREB3)was focused.Evolution of its protein encoded by AhDREB3 was analyzed.Primers were designed to detect its expression under several abiotic stresses by fluorescence quantitative PCR.Results showed that AhDREB3 gene did not respond to low temperature in leaves and roots of peanut,but responded significantly to high salt(in leaves and roots)and drought(in roots)stress.In addition,the AhDREB3 gene did not respond significantly to exogenous ABA in peanut leaves and roots,suggesting that the gene might play a role in peanut through an ABA-independent pathway.