接种根瘤菌环境下大豆叶形遗传及QTL定位分析

Genetic analysis and QTL mapping of soybean leaf shape under rhizobia inoculated environment

为探究接种根瘤菌环境下影响大豆叶形的遗传基础,本研究利用两个叶形具有显著差异的大豆品种及RIL群体,在接种和不接种根瘤菌环境下对大豆叶形性状进行遗传和QTL定位等分析。结果显示,叶形相关性状的遗传率在0.60~0.95之间,环境与基因型间存在互作效应,并且接种根瘤菌可以显著影响叶形指数(LS)与单株粒数(GN)、单株荚数(PD)和单株粒重(GW)的相关系数。此外,两种处理下共检测到8个QTL位点,LOD值范围在2.50~7.03,可解释6.4%~16.9%的遗传变异。其中,qLS-15可解释由根瘤菌×基因型互作引起叶形性状6.4%~9.3%的遗传变异,LOD值在2.50~3.69之间,表明qLS-15是与环境互作的主要遗传位点之一。综上所述,根瘤菌可以通过qLS-15影响大豆叶形,研究结果为解析根瘤菌提升大豆产量的内在机制提供了理论依据。

To determine the genetic basis of soybean leaf shape under the environment with rhizobia inoculation,two soybean varieties with contrast leaf shape and their derived RIL population were used for genetic and QTL analysis under both environment with and without rhizobia inoculation.As results showed that the heritability of leaf shape related traits varied from 0.60 to 0.95 and there also existed interactions between environment and genotype,meanwhile,rhizobia inoculation could significantly affect the correlation coefficients of leaf shape index(LS)with grain number(GN),pod number(PN)and grain weight(GW)per plant.In addition,total 8 QTL with LOD values varied from 2.50~7.03,which could explain 6.4%~16.9%genetic variation,were determined under both environments with and without rhizobia inoculation.Among them,qLS-15 with LOD values varied from 2.50~3.69 could explain the 6.4%~9.3%genetic variation caused by rhizobia*genotype interaction for leaf shape traits,suggesting that qLS-15 is one of the main genetic loci that could interact with the environment.In summary,rhizobia could affect soybean leaf shape through genetic locus of qLS-15,these results provide a theoretical basis for analyzing the mechanism of rhizobia in increasing soybean yield.