外周血单个核细胞中circTCF25表达水平在肺源性心脏病患者诊断及预后中的应用

Application of circTCF25 in Peripheral Blood Mononuclear Cells on Diagnosis and Prognosis Evaluation of Cor Pulmonale

目的探讨外周血单个核细胞(PBMCs)中环状RNA circTCF25在肺源性心脏病(CP)患者诊断及预后评估中的应用价值。方法选取2016年11月—2018年11月西安市第九医院收治的CP患者52例(CP组),另选取同期性别、年龄匹配的健康体检者30例(对照组)作为研究对象,采用实时荧光定量聚合酶链反应检测两组PBMCs中circTCF25表达水平;采用Pearson相关法对CP组circTCF25水平与动脉血氧分压(PaO_(2))、脑钠肽(BNP)进行相关性分析;应用受试者操作特征(ROC)曲线评价circTCF25在CP诊断和预后评估中的应用价值。结果CP组PaO_(2)水平显著低于对照组(P<0.05),BNP水平显著低于对照组(P<0.05);CP组PMBCs中circTCF25水平显著高于对照组(P<0.05);Pearson相关分析结果显示circTCF25表达水平与PaO_(2)呈显著负相关(r=0.633,P<0.05),与BNP水平呈显著正相关(r=0.528,P<0.05);ROC曲线分析结果显示,circTCF25诊断CP的最佳截值为1.37,ROC曲线下面积(AUC)为0.834(95%CI 0.734~0.908);circTCF25预测CP患者预后不良的最佳截值为1.74,预测的AUC为0.722(95%CI 0.636~0.798)。结论PBMCs中circTCF25表达水平在CP患者明显上调,其对CP诊断和预后评估具有较好的应用价值。

Objective To explore the application value of circular RNA TCF25(circTCF25)in peripheral blood mononuclear cells(PBMCs)on diagnosis and prognosis evaluation of cor pulmonale(CP).Methods 52 patients with CP(CP group)and 30 healthy controls(control group)with matched gender and age admitted to our hospital from November 2016 to November 2018 were enrolled as research objects.The expression level of circTCF25 in PBMCs was detected by RT-PCR.Correlations of circTCF25 with arterial oxygen partial pressure(PaO_(2))and brain natriuretic peptide(BNP)in CP group were analyzed with Pearson correlation analysis.The application value of circTCF25on diagnosis and prognosis evaluation of CP was determined by receiver operating characteristic(ROC)curve.Results Compared with control group,PaO_(2) and BNP level in CP group were both decreased(P<0.05),and circTCF25 level in PMBCs was increased(P<0.05).Pearson correlation analysis showed that circTCF25 level was negatively correlated with PaO_(2)(r=0.633,P<0.05),and positively correlated with BNP level(r=0.528,P<0.05).ROC curves analysis showed that the best cut-off value of circTCF25 for diagnosis with CP was 1.37,with the area under ROC curve(AUC)of 0.834(95%CI 0.734~0.908).The best cut-off value and AUC of circTCF25 for predicting poor prognosis of CP were 1.74 and 0.722(95%CI 0.636~0.798),respectively.Conclusion The expression level of circTCF25 in PBMCs is distinctly upregulated in CP patients,which may be of good application value on diagnosis and prognosis evaluation for CP.