单丛茶HS-SPME/GC-MS指纹图谱的构建

Construction of Fingerprint of Dancong Tea Using HS-SPME/GC-MS

对单丛茶顶空固相微萃取的萃取时间、温度和茶叶比等条件进行优化研究,并对各个样品进行气相色谱-质谱(GC-MS)检测和分析,建立单丛茶的挥发性物质指纹图谱。研究表明,最佳萃取条件为0.75:5 g/mL的茶水比,在90℃温度下萃取50 min。建立的单丛茶挥发性物质指纹图谱具有20个共有峰,图谱相似度达0.9以上。不同地区单丛茶的判别模型具有较好的准确性,其自身验证法和交叉验证法的正确率均为100%,外部验证法正确率为80%。该研究结果可为单丛茶顶空固相微萃取研究提供试验依据,为单丛茶鉴别提供理论参考。

The extraction conditions for volatile compounds of Dancong tea by headspace solid phase micro-extraction,including the extraction time,the temperature and the ratio of tea and water,were optimized.Each sample was detected and analyzed by gas chromatography-mass spectrometry(GC-MS)to establish the volatile substance fingerprint of Dancong tea.The results showed that the optimal extraction conditions were 0.75:5 g/mL tea-water ratio,and extraction was performed at 90℃for 50 min.The volatile substances fingerprints of Dancong tea identified 20 common peaks and the similarity was above 0.9.The discriminant model had good accuracy for the classification of Dancong tea in different regions.The accuracy rate of its own verification method and the interactive verification method were 100%while the external verification method was 80%.The results can provide experimental basis for the headspace solid phase microextraction of Dancong tea and provide a theoretical reference for the identification of Dancong tea.