摘要
建立了浊点萃取-气相色谱法同时测定6种有机磷农药(OPPs)的分析方法。以聚乙二醇6000为萃取剂,乙酸乙酯和正己烷混合液(65∶35)作为反萃取剂,兼顾不同极性OPPs的萃取,并解决了表面活性剂因沸点高、粘度大无法进入气相检测的问题。在最佳条件下,6种OPPs在2.5~8000μg/L范围与峰面积呈现良好的线性关系(r2≥0.9958),检出限在0.8~5.0μg/L范围内,相对标准偏差(RSD)<5.18%(n=6)。该方法成功应用于测定环境水样和土壤中的6种OPPs,加标回收率为89.7%~109.9%。
A novel method termed cloud point extraction combined with gas chromatography was established for simultaneous determination of 6 organophosphorus pesticides(OPPs)with different polarities.Polyethylene glycol 6000 was used as the extractant,and a mixture of ethyl acetate and n-hexane(65∶35,V/V)was used as the back extraction agent.This method took into account the extraction of different polarities organophosphorus pesticides,and solved the issues that the surfactants could not be directly introduced to gas chromatography for detection because of its high boiling point and high viscosity.Under the optimum conditions,good linearity(r2≥0.9958)was obtained in the range of 2.5-8000μg/L.The low limits of detection were between 0.8μg/L and 5.0μg/mL,and the relative standard deviations were below 5.18%(n=6).The method has been successfully applied to the determination of 6 OPPs in environmental water and soil samples with good recoveries of 89.7%-109.9%.
作者
谢美仪
姜勋
刘炜婷
郝晓棠
刘甜恬
郑涵
王梅
XIE Meiyi;JIANG Xun;LIU Weiting;HAO Xiaotang;LIU Tiantian;ZHENG Han;WANG Mei(School of Public Health,GuangdongPharmaceutical University,Guangzhou510310)
出处
《分析科学学报》
CAS
CSCD
北大核心
2021年第5期675-679,共5页
Journal of Analytical Science
基金
广东省重点领域研发计划(No.2020B111106001)
广州市科技计划(No.202002030409)
广东药科大学"创新强校工程"项目(No.2020KZDZX1122,2019GCZX012)。
关键词
有机磷农药
浊点萃取
气相色谱法
农药残留
反萃取
OPPs
Cloud point extraction
Gas chromatography
Pesticide residue
Back extraction