黄连水提液对腐皮镰孢菌抑制效果研究

Study on Inhibitory Effect of the Water Extract of Coptis chinensis on Fusarium solani

[目的]探究黄连水提液对腐皮镰孢菌(Fusarium solani)的抑菌效果,为深入研究中药对植物根腐病防治提供科学参考。[方法]将黄连剪成0.5 cm左右的小段,称取10 g黄连,加入150mL无菌水煮沸,小火熬30 min,提取其有效成分,得黄连水提液。采用二倍稀释法和菌丝生长速率法测定黄连水提液对腐皮镰孢菌的最小抑菌浓度及最适抑菌量。[结果]1g/mL黄连水提液稀释后对腐皮镰孢菌的抑菌效果较差,4 d平均抑菌率不到20%,最小抑菌浓度为16 mg/mL;1g/mL的黄连水提液增加用量后对腐皮镰孢菌的抑制效果明显增加,最适用量为0.3mL,此时最大抑菌率可达76.56%,最大平均抑菌率达57%。[结论]不同浓度及不同用量黄连水提液对腐皮镰孢菌的菌丝生长具有显著影响,最小抑菌浓度为16 mg/mL,最适抑菌量为0.3mL。

[Objective]To investigate the antibacterial effect of water extract of C.chinensis on F.solani,and to provide scientific reference for further research on the prevention and treatment of plant root rot.[Method]C.chinensis was cut into small pieces about 0.5 cm, weighed 10 g of C.chinensis,added 150 mL sterile water to boil for 30 min at low fire, and the effective components were extracted to obtain water extract of C.chinensis.The minimum inhibitory concentration and the optimal inhibitory amount of water extract of C.chinensis against F.solani were determined by double dilution method and mycelial growth rate method.[Result]The antibacterial effect of 1 g/mL water extract of C.chinensis on F.solani was poor.The average antibacterial rate of 4 d was less than 20%,and the MIC value was 16 mg/mL.When the dosage of water extract of 1 g/mL of C.chinensis was increased, the inhibition effect on F.solani was significantly increased.The most suitable dosage was 0.3 mL,and the maximum and average inhibition rate reached 76.56% and 57% at this time.[Conclusion]The different concentrations and dosages of water extract of C.chinensis have significant effects on the mycelial growth of F.solani.The MIC is 16 mg/mL,and the optimal bacteriostatic amount is 0.3 mL.