基于液相芯片筛查与肝癌相关血清自身抗体的研究

Study on liquid-chip-based screening of hepatocellular carcinoma associated serum autoantibody

目的筛查与肝癌(HCC)相关的自身抗体并对其诊断肝癌的临床价值进行初步评估。方法制备用于自身抗体多重检测的液相芯片,对40例肝炎、32例肝硬化、48例肝癌及30例健康对照组血清进行检测,比较自身抗体IgG水平,筛选与肝癌相关的自身抗体,绘制ROC曲线评估其肝癌的诊断价值,并分析肝癌相关自身抗体与常见生化指标的相关性。结果肝炎、肝硬化、肝癌组的SSA/Ro、tTG、LC1、PR3、Scl-70、Mi-2、MPO和TIF1-γ自身抗体水平均显著高于正常对照组(P<0.05)。ROC曲线显示SSA/Ro、tTG、LC1、PR3、Scl-70、Mi-2和SP100的自身抗体对肝癌诊断的曲线下面积均高于肝癌的标志物AFP。相关性分析显示,肝癌相关自身抗体之间普遍呈正相关性,而与生化指标无显著相关性。结论筛选到SSA/Ro、tTG、LC1、PR3、Scl-70、Mi-2和SP100自身抗体在肝炎、肝硬化、肝癌中存在异常表达,提示可能与肝癌的发生发展有关。

Objective To investigate the clinical significance of serum autoantibodies in patients with hepatocellular carcinoma(HCC).Methods The values of serum autoantibodies in 40patients with hepatitis,32patients with liver cirrhosis,48patients with HCC and 30healthy controls were detected simultaneously using the Luminex xMAP liquid chip technology.These autoantibodies were further evaluated the clinical significance for diagnosing HCC using the ROC curve analysis and Spearman test.Results The serum levels of anti-SSA/Ro,anti-tTG,anti-LC1,anti-PR3,anti-Scl-70,anti-Mi2,anti-MPO and anti-TIF1-γin liver diseases including hepatitis,liver cirrhosis and HCC were significantly higher than those in healthy controls(P<0.05).ROC analysis showed that anti-SSA/Ro,anti-tTG,anti-LC1,anti-PR3,anti-Scl-70,anti-Mi-2and anti-SP100superior to AFP for diagnosing HCC with a larger area under curve(AUC).In addition,positive correlation was observed between different autoantibodies,but there was no significant correlation between autoantibodies and biochemical indexes.Conclusion The serum levels of anti-SSA/Ro,anti-tTG,anti-LC1,anti-PR3,anti-Scl-70,anti-Mi-2and anti-MPO were abnormally in the patients with hepatitis,liver cirrhosis and HCC,which might be important for the diagnosis of HCC,provide theoretical basis in the genesis and development of HCC.