分蘖洋葱鳞茎中黄酮类化合物分析及其与番茄根结线虫病发生的关系

Analysis of flavonoids in potato onion bulbs and its relationship with tomato root knot nematode

分蘖洋葱伴生可减轻番茄根结线虫病,但是否与分蘖洋葱鳞茎中黄酮类化合物有关尚不清楚。以南方根结线虫和44份分蘖洋葱种质为试验材料,研究了44份分蘖洋葱鳞茎中黄酮类化合物(芦丁、杨梅黄酮、山奈酚、槲皮素及总黄酮)含量及其对番茄根结线虫死亡率、虫卵孵化抑制率以及线虫趋化性Cf值的影响,同时分析了黄酮类化合物与根结线虫发病率、病情指数、土壤虫口防效以及根结指数间相关性。结果表明,44份分蘖洋葱鳞茎黄酮类化合物含量为593.22~2204.88 mg·100 g^(-1)DW,其中M11和M51鳞茎中黄酮类化合物含量较高,为2186.59和2204.88 mg·100 g^(-1)DW,以槲皮素为主;分蘖洋葱鳞茎中杨梅黄酮、槲皮素、山奈酚及总黄酮含量均与根结线虫虫卵孵化抑制率呈显著正相关,黄酮类化合物含量均与根结线虫趋化性Cf值呈显著负相关,杨梅黄酮与土壤虫口防效呈显著正相关,与番茄根结线虫病情指数和根结指数呈显著负相关;槲皮素与番茄根结指数、根结线虫病情指数和发病率呈显著负相关。综上,分蘖洋葱伴生减轻番茄根结线虫病,与分蘖洋葱鳞茎中黄酮类化合物含量密切相关。

Companion with potato onion can suppress tomato root-knot nematode disease.However,whether flavonoids in potato onion bulb could inhibit tomato root-knot nematode disease remains unclear.In this study,the contents of flavonoids(rutin,myricetin,kaempferol,quercetin and the total flavonoids)from 44 potato onion bulbs and their effects on tomato root-knot mortality,egg hatching inhibition rate and Cf value of chemotaxis were investigated.Moreover,the correlations between flavonoids and root-knot nematode incidence rate,disease index,soil insect control effect and root-knot index were also assessed.The results found that the contents of flavonoids from 44 potato onion bulbs were ranged from 593.22 to 2204.88 mg·100 g^(-1) DW.Among them,the contents of flaconoids were higher in M11 and M51 mainly with quercetin as the main compounds,and the values are 2186.59 and 2204.88 mg·100 g^(-1) DW,respectively.The contents of myricetin,quercetin,kaempferol and total flavonoids in potato onion bulbs were significantly positively correlated with the egg hatching inhibition rate of root-knot nematode,and the content of flavonoids were significantly negatively correlated with the Cf value of root-knot nematode chemotaxis.In addition,myricetin was significantly positively correlated with soil insect control effect,and significantly negatively correlated with disease index and root-knot index of tomato root-knot nematode.Quercetin was significantly negatively correlated with tomato root knot index,root knot nematode disease index and incidence.Overall,companion with potato-onion can reduce root-knot nematode of tomato,which closely related to the contents of flavonoids in potato onion.