硒对铅中毒诱导蛋鸡肾脏炎症损伤缓解作用研究

Effects of selenium relieving lead-induced inflammation in kidneys of laying hens

为研究硒缓解铅致鸡肾脏炎症损伤的作用,试验将72只海兰白蛋鸡随机分为四组,分别饲喂标准日粮和饮用水、添加1 mg·kg-1硒的日粮和标准饮用水、标准日粮和添加350 mg·L^(-1)铅的水、添加1 mg·kg^(-1)硒的日粮和350 mg·L^(-1)铅的水。第4、8和12周测定肾脏中硒(Se)和铅(Pb)浓度、血清中肌酐(CREA)和尿酸(UA)含量、氧化应激指标、细胞因子和炎症因子mRNA和蛋白质相对表达量。结果表明,加铅组蛋鸡肾脏发生损伤,同时铅含量增加、硒含量降低,CREA和UA含量增加;过量铅引起蛋鸡肾脏中MDA含量,IL-1β、IL-6、IL-17、caspase-1、TNF-α、NF-κB、NLRP3、COX-2表达量增加、GSH含量降低、GPx和SOD活性降低及IFN-γ表达量降低;硒缓解过量Pb中毒引起的上述指标变化。从相对表达量看,NLRP3炎症小体、caspase-1和IL-1β可能是铅诱导蛋鸡肾炎症损伤的敏感指标。

To study the mechanism of mitigative role of Se on Pb-caused renal inflammatory injury in chickens.The fully randomized design was conducted,72 chickens were divided into four groups randomly and provided a standard diet and drinking water,a standard diet added with 1 mg·kg^(-1) of Se and drinking water,a standard diet and drinking water added with 350 mg·L^(-1) Pb,a standard diet added with 1 mg·kg^(-1) Se,and drinking water added with 350 mg·L^(-1) Pb.On the 4th,8th and 12th weeks,the concentration of selenium(Se)and lead(Pb)in the kidney,the contents of creatinine(CREA)and uric acid(UA)in the serum,the oxidative stress indicators,the relative expression of cytokines and inflammatory factors mRNA and protein were measured.The results showed that Pb accumulated,Se concentration decreased,CREA and UA contents increased,and renal injury occurred in Pb-treated chicken kidneys.Excess Pb increased MDA content,the expressions of IL-1β,IL-6,IL-17,caspase-1,TNF-α,NF-κB,NLRP3,COX-2;and decreased GSH content,GPx and SOD activities and IFN-γexpression in kidneys of laying hens.Se administration alleviated the aforementioned changes.NLRP3 inflammasome,caspase-1 and IL-1βmight be sensitive indicators in Pb-induced chicken kidney inflammatory damage.