高碘膳食干预人群血清差异代谢生物标志物研究

Serum biomarkers of differential metabolism in high iodine diet intervention population

目的探索高碘膳食干预人群血清差异代谢产物。方法在哈尔滨医科大学招募38名健康志愿者,全部为女性,年龄>20岁。采用进食富碘食品(海带)的方式进行人群膳食干预,连续干预11 d。通过尿碘检测评价干预效果;并采集外周血,应用超高效液相色谱与四级杆飞行时间串联质谱(UPLC-Q-TOF MSMS)分析干预前后血清代谢产物,应用多元统计分析筛选潜在的生物标志物,并进行扰动代谢通路分析。结果干预前后人群尿碘中位数分别为129.48、795.94μg/L。共筛选出20个血清差异代谢生物标志物并进行了物质的化学结构鉴定。甘油磷脂代谢通路、色氨酸代谢通路、戊糖和葡萄糖醛酸转换通路与高碘膳食密切相关(影响值>0.1)。结论人群高碘膳食干预后,有明显的外周血差异代谢产物,这些差异代谢产物有可能用于群体碘营养评价的生物标志物;高碘摄入对人体的甘油磷脂代谢通路产生了影响。

Objective To explore the serum different metabolites in population of dietary intervention with high iodine.Methods Thirty-eight healthy volunteers were recruited in Harbin Medical University,all women,aged>20 years.Dietary intervention was carried out by eating iodine-rich food(kelp)for 11 consecutive days.The effect of intervention was evaluated through urinary iodine test.Peripheral blood was collected,the metabolic alterations associated with high iodine intake before and after intervention were investigated using ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry(UPLC-Q-TOF MSMS).Multivariate statistical analysis was used to screen potential biomarkers.And disturbed metabolic pathway analysis was performed.Results The medians urinary iodine before and after intervention were 129.48 and 795.94μg/L,respectively.A total of 20 serum biomarkers were screened and their chemical structures were identified.Glycerol phospholipid metabolic pathway,tryptophan metabolic pathway and pentose and glucuronate interconversions pathway were closely related with high iodine intake(impact value>0.1).Conclusions After the intervention of high iodine diet,there are obvious differential metabolites in peripheral blood,which may be used as biomarkers for evaluation of population iodine nutrition.High iodine intake has an impact on metabolic pathway of glycerol phospholipid in human body.