异麦芽酮糖及其生产方式的研究进展

Research progress of isomaltulose and its production methods

随着人们对健康生活重视程度的不断提升,寻求健康型甜味剂以满足消费者的需求显得至关重要。异麦芽酮糖是一种安全的蔗糖替代品,具有水解速度慢、低GI(血糖生成指数)、抗龋齿和益生元特性等生理功能,已应用于巧克力、谷类早餐、冰淇淋等各类食品中。异麦芽酮糖主要通过蔗糖异构酶转化蔗糖生成,反应中的副产物给工业生产带来困扰。综述了异麦芽酮糖的物理特性和生物活性,系统阐述近年来异麦芽酮糖的生产方式——微生物转化法和蔗糖异构酶催化法,包括菌体发酵法、固定化细胞、固定化酶及新兴的表面展示系统等技术的生产工艺及生产中存在的问题。详细介绍了微生物转化法中改造菌株的方式及优缺点,酶催化合成法的应用前景及限制因素,为异麦芽酮糖的工业化生产提供参考。

As people attach great importance to healthy life,it is very important to seek healthy sweeteners to meet the needs of consumers.Isomaltulose is a safe sucrose substitute and a natural isomer of sucrose.It has physiological functions such as slow hydrolysis,low GI value(glycemic index),anti-caries,islet protection,and prebiotic properties.As a new food additive,it has been used in chocolate,cereal breakfast,ice cream and other foods.Isomaltulose is mainly produced through the conversion of sucrose by sucrose isomerase,and the by-products of the reaction bring trouble to industrial production.The physical characteristics and biological activities of isomaltulose were reviewed,and the production methods of isomaltulose in recent years,such as microbial transformation and sucrose isomerase catalysis,including bacterial fermentation,immobilized cells,immobilized enzyme and the emerging surface display system,and the problems existed in the production were systematically described.In this review,the methods of bacterial strain modifications and their advantages and disadvantages were introduced in detail,respectively.The application prospect and limiting factors of the enzyme-catalyzed synthesis method were also introduced,so as to provide a reference for the industrial production of isomaltulose.