斑鞘豆叶甲成虫对大豆叶片挥发物的触角电位和行为反应

Electroantennogram and Behavioral Responses of Colposcelis signata Adults to Volatiles from Soybean Leaves

利用触角电位仪(EAG)和Y型嗅觉仪从8种大豆叶片挥发物中筛选出对斑鞘豆叶甲成虫具有显著引诱或驱避作用的化合物,为探索生物制剂防控斑鞘豆叶甲提供理论依据。EAG测定结果表明:斑鞘豆叶甲EAG反应相对值均随挥发物浓度的增加而增大,且雌虫强于雄虫。同一浓度下,对1-辛烯-3-醇的EAG反应相对值最大,其次为3-辛醇和(Z)-3-己烯-1-醇。行为反应测试发现,同一浓度的不同挥发物对斑鞘豆叶甲的引诱率或驱避率间存在差异。1-辛烯-3-醇、3-辛醇、(Z)-3-己烯-1-醇、3-辛酮、茉莉酸甲酯和N,N-二甲基乙酰胺对斑鞘豆叶甲均有显著的引诱作用,并随着浓度的增大引诱率增加,且雌虫的嗅觉反应均强于雄虫。其中10^(-1) mol/L的1-辛烯-3-醇对雌、雄虫的引诱率最高,分别为90.00%和84.45%。己醛的驱避率最高,显著高于其他挥发物。β-石竹烯在低浓度(10^(-5)~10^(-4)mol/L)下具有显著的引诱作用,在高浓度(10^(-2) ~10^(-1)mol/L)下具有显著的驱避作用。

In this study, electroantennogram(EAG) and Y tube olfactomcter were used to screen the attractive and repellent compounds from 8 kinds of volatiles from soybean leaves on Colposcelis signata adults to provide theoretical basis for exploring biological agents to prevent this pest. EAG bioassay results showed that the relative values of EAG response increased with the increase of volatile concentration and the female was stronger than the male. At the same concentration, the relative value of EAG response for 1-octene-3-ol was the largest, followed by 3-octanol and(Z)-3-hexene-1-ol. As for behavioral response, there were differences among lure rate or repellent rate of different volatiles at the same concentration. 1-octen-3-alcohol, 3-octanol,(Z)-3-hexen-1-ol, 3-octanone, methyl jasmonate and N, N-dimethyl cacetamide had significant attractive effects. With the increase of concentration, lure rate increased. 1-octene-3-ol at 10^(-1) mol/L was the most attractive to females and males, which were 90.00% and 84.45%, respectively. Repellent rate of hexanal was the highest, which was significantly higher than that of other plant volatiles. β-caryophyllene at low concentration of 10^(-5)-10^(-4)mol/L could attract females and males, but at high concentration of 10^(-2) -10^(-1) mol/L, it had significant repellent effect.