豆渣源纳豆激酶制剂喷雾干燥工艺优化及其稳定性

Optimization and Stability of Spray Drying Process of Nattokinase Preparation from Soybean Dregs

优化豆渣为原料生产纳豆激酶的喷雾干燥工艺并探究纳豆激酶的稳定性。在单因素试验基础上,应用Box-Behnken中心组合设计原理,设计保护剂种类、进口温度、蠕动泵转速三因素三水平响应面试验,建立回归模型。通过响应面分析,回归模型具有较高的拟合度。优化后的喷雾干燥工艺参数:保护剂类别为海藻糖,进口温度152℃,蠕动泵转速378 mL/h,优化后纳豆激酶活性为236 278 FU/g。稳定性试验结果表明:在0℃、4℃、常温(18℃)、37℃的干燥保藏条件下,喷雾干燥后纳豆激酶活力均较为稳定,随着时间的增长,纳豆激酶活力逐渐降低,其中以海藻糖为保护剂的纳豆激酶稳定性最高。

The spray drying process of nattokinase from fermented soybean dregs was optimized and its stability was explored. On the basis of single-factor test, three independent variables(protective category, inlet temperature and peristaltic pump speed) were selected using Box-Behnken to design a response surface experiment, which constructs a regression model with high degree of fitting. The parameters of the optimized spray drying process are as follows: protective category is trehalose, inlet temperature is 152 ℃, peristaltic pump speed is 378 mL/h. Under the conditions, nattokinase activity can reach 236 278 FU/g. Nattokinase preserved as dry at the temperature of 0 ℃, 4 ℃, room temperature(18 ℃) and 37 ℃ had a relative stable activity after spray drying, while the activity gradually reduced as the time extending. The stability of nattokinase was the highest when trehalose was used as a protective agent.