中华管鞭虾中虾青素的肠吸收特性研究

Study on intestinal absorption characteristics of astaxanthin extracted from Solenocera crassicornis

对Sprague Dawley雄性大鼠进行单次大剂量灌胃皂化和未皂化虾青素,采用高效液相色谱法分析灌胃6 h和12 h后小肠不同部位内容物中虾青素含量,并检测连续灌胃14 d后大鼠新鲜粪便中虾青素总量、游离型和酯型虾青素含量。结果表明,灌胃6 h后,大鼠空肠、回肠和结肠内容物中均未检测到虾青素;灌胃12 h后,皂化和未皂化虾青素组在结肠内容物中均检测出虾青素,且皂化组虾青素总量(283.221±89.50)μg/g显著高于未皂化组(126.850±40.49)μg/g;连续灌胃14 d后,大鼠粪便中皂化组虾青素的残留量显著低于未皂化组,且主要为游离型虾青素(11.12±0.36)μg/g,而未皂化虾青素组大鼠粪便中游离型和酯型虾青素含量分别为(21.78±3.40)μg/g和(7.69±0.32)μg/g。因此,皂化后中华管鞭虾的虾青素提取物较未皂化的虾青素提取物有更好的肠吸收性。

The Sprague Dawley male rats were given a single high-dose gavage with saponified and unsaponified astaxanthin,the con⁃tent of astaxanthin in different parts of the small intestine after 6 h and 12 h were determined,and the total content of astaxanthin,free astaxanthin and ester astaxanthin in fresh feces of rats after 14 d of continuous gavage were detected by HPLC.The results showed that after 6 h of gavage,astaxanthin was not detected in the jejunum,ileum and colon of rats.After 12 h of gavage,astaxanthin was detect⁃ed in the colon contents of both the saponified and unsaponified astaxanthin group,and the content of astaxanthin in the saponified group(283.221±89.50μg/g)was significantly higher than that in the unsaponified group(126.850±40.49μg/g).After 14 d of continu⁃ous gavage,the astaxanthin residue from in feces of rats in the saponified group was significantly lower than that in the unsaponified group,and the astaxanthin residue was mainly composed of free astaxanthin(11.12±0.36μg/g).However,the contents of free astaxan⁃thin and ester astaxanthin in the rats feces of unsaponified group were 21.78±3.40μg/g and 7.69±0.32μg/g,respectively.Therefore,the saponified astaxanthin derived from Solenocera crassicornis showed better intestinal absorption compared with the unsaponified astaxanthin.