妊娠期糖尿病患者外周血SREBP-1、PPARα表达与胰岛素抵抗关系

Relationship between the expressions of sterol regulatory element binding protein-1 and peroxisome proliferator-activated receptorαof women with gestational diabetes mellitus and their insulin resistance

目的:检测妊娠期糖尿病(GDM)患者外周血固醇调节元件结合蛋白-1(SREBP-1)、过氧化物酶体增殖物激活受体α(PPARα)表达,并探讨其与胰岛素抵抗关系。方法:选取2018年2月-2020年6月在本院定期产前检查的GDM患者90例(GDM组),正常妊娠孕妇90例(对照组)。实时荧光定量PCR法检测外周血SREBP-1、PPARαmRNA表达水平;Western Blot法检测外周血SREBP-1、PPARα蛋白表达水平;全自动电化学发光免疫分析仪检测空腹胰岛素(FINS)、空腹血糖(FPG)水平并计算胰岛素抵抗指数(HOMA-IR);Pearson法分析外周血SREBP-1、PPARαmRNA及蛋白表达水平与HOMA-IR水平的相关性。结果:与对照组相比,GDM组外周血SREBP-1 mRNA及蛋白表达水平,FINS、FPG、HOMA-IR水平均升高,外周血PPARαmRNA及蛋白表达水平均降低(均P<0.05);GDM孕妇外周血SREBP-1 mRNA、SREBP-1蛋白水平与HOMA-IR水平均呈正相关(P<0.05),外周血PPARαmRNA、PPARα蛋白水平与HOMA-IR水平均呈负相关(P<0.05)。结论:GDM患者外周血SREBP-1升高、PPARα降低,均与胰岛素抵抗呈一定相关性,其可能通过调节患者体内胰岛素抵抗水平参与GDM发生。

Objective:To detect the expressions of sterol regulatory element binding protein-1(SREBP-1)and peroxisome proliferator-activated receptorα(PPARα)in peripheral blood of pregnant women with gestational diabetes mellitus(GDM),and to explore the relationship between them and insulin resistance.Methods:90 pregnant women with GDM were selected in study group and 90 normal pregnant women were selected in control group from February 2018 to June 2020.The mRNA levels of SREBP-1 and PPARαin peripheral blood of the women in the two groups were detected by real time fluorescent quantitative PCR(qRT-PCR),and the protein levels of SREBP-1 and PPARαin peripheral blood of the women in the two groups were detected by Western Blot.The levels of fasting insulin(FINS)and fasting blood glucose(FPG)were detected by automatic electrochemiluminescence immunoassay,and the insulin resistance index(HOMA-IR)was calculated.The correlation between mRNA and protein expression levels of SREBP-1 and PPARαin peripheral blood of the women and their HOMA-IR value was analyzed by Pearson method.Results:The mRNA and protein expression levels of SREBP-1,the levels of FINS and FPG,and HOMA-IR value of the women in the study group were significant higher than those of the women in the control group,but the mRNA and protein levels of PPARαof the women in the study group were significant lower(all P<0.05).The mRNA and protein levels of SREBP-1 of the women in the study group were positively correlated with their HOMA-IR value(P<0.05),and the mRNA and protein levels of PPARαof the women in the study group were negatively correlated with their HOMA-IR value(P<0.05).Conclusion:The SREBP-1 level in the peripheral blood of the women with GDM increases significantly,but the PPARαlevel decreases significantly,both of which are related to the insulin resistance of the women,and they maybe participate in the occurrence of GDM by regulating the insulin resistance of the women.