DNMT3B、MMP-2和ENA-78在子宫内膜异位组织中表达及相关性

Expression of DNA methyltransferase 3B,matrix metalloproteinase-2,and epithelial neutrophil-activating peptide 78 in endometriosis tissue of women and their correlation with the endometriosis occurrence

目的:探究子宫内膜异位组织中DNA甲基化转移酶3B(DNMT3B)、基质金属蛋白酶-2(MMP-2)和上皮来源中性粒细胞活化肽(ENA-78)的表达及相关性。方法:选择2017年3月—2020年10月在本医院就医的子宫内膜异位症患者51例为异位症组,37例行绝育手术的健康女性为对照组,免疫组化法检测异位症组异位内膜、在位内膜和对照组正常子宫内膜中DNMT3B、MMP-2和ENA-78的表达,并行相关性分析和诊断效能分析。结果:异位症组异位内膜表达阳性率DNMT3B(84.3%)、MMP-2(90.2%)、ENA-78(80.4%)均高于在位内膜和对照组正常子宫内膜(P<0.05);异位症组异位内膜中,MMP-2与DNMT3B、ENA-78均呈正相关(P<0.05);DNMT3B、MMP-2、ENA-78联合检测诊断子宫内膜异位症的AUC为0.924,准确度(92.0%)均高于各单独指标检测(75.0%、76.1%、72.7%)(P<0.05)。结论:DNMT3B、MMP-2、ENA-78在异位内膜中的阳性率增加,3项指标联合检测可为子宫内膜异位症诊断提供参考。

Objective:To explore the expression of DNA methyltransferase 3B(DNMT3B),matrix metalloproteinase-2(MMP-2),and epithelial neutrophil-activating peptide 78(ENA-78)in endometriosis tissue of women,and to study their correlation with the endometriosis occurrence.Methods:51 women with endometriosis were selected in study group and 37 healthy women were selected in control group from March 2017 to October 2020.Immunohistochemistry was used to evaluate the expressions of DNMT3B,MMP-2,and ENA-78 in ectopic and eutopic endometrial tissues of the women in the study group and in normal endometrial tissues of the women in the control group.The correlation analysis and diagnostic efficacy analysis were performed.Results:The expression positive rate of DNMT3B(84.3%),MMP-2(90.2%),and ENA-78(80.4%)in ectopic endometrium of the women in the study group were significant higher than those in eutopic endometrial tissues of the women in the study group and in normal endometrial tissues of the women in the control group(P<0.05).In ectopic endometrium of the women in the study group,the MMP-2 expression were positively correlated with the expressions of DNMT3B and ENA-78(P<0.05).AUC of the combination detections of DNMT3B,MMP-2,and ENA-78 for diagnosing endometriosis was 0.924,which accuracy(92.0%)were significant higher than that(75.0%)of DNMT3B alone,that(76.1%)of MMP-2 alone,or that(72.7%)of ENA-78 alone(P<0.05).Conclusion:The positive rates of DNMT3B,MMP-2,and ENA-78 in ectopic endometrium of the women with endometriosis increase significantly,and the combination detections of DNMT3B,MMP-2,and ENA-78 can provide evidences for diagnosing endometriosis.