三阴性乳腺癌与非三阴性乳腺癌组织中PD-1、PD-L1、FOXP3的差异表达及其临床意义

Differential Expression and Clinical Significance of PD-1,PD-L1 and FOXP3 in Triple-negative Breast Cancer and Non-triple-negative Breast Cancer Tissues

目的探讨三阴性乳腺癌患者组织中程序性死亡分子-1(PD-1)、程序性死亡分子配体-1(PD-L1)、叉状头/翅膀状螺旋转录因子(FOXP3)的表达及其临床意义。方法选取2015年1月-2020年1月我中心诊断的70例三阴性乳腺癌,30例非三阴性乳腺癌患者为研究对象,采用免疫组化法测定三者中PD-1、PD-L1、FOXP3表达情况,分析三阴性乳腺癌与非三阴性乳腺癌组织中PD-1、PD-L1、FOXP3的差异表达及与患者临床特征的相关性。结果三阴性乳腺癌中PD-1、PD-L1、调节性T淋巴细胞FOXP3阳性表达率均高于非三阴性乳腺癌(P<0.05);三阴性乳腺癌组织中PD-1、PD-L1阳性表达率与患者年龄、肿瘤直径无显著关联性(P>0.05);Ⅲ级、淋巴脉管侵犯及淋巴结转移患者三阴性乳腺癌组织中PD-1、PD-L1阳性表达率分别高于Ⅰ、Ⅱ级、无淋巴血管侵犯及无淋巴结转移患者(P<0.05);三阴性乳腺癌组织中FOXP3阳性表达率与患者年龄、肿瘤直径、有无淋巴结转移无显著关联性(P>0.05);Ⅲ级及淋巴血管侵犯患者三阴性乳腺癌组织中调节性T淋巴细胞FOXP3阳性表达率分别高于Ⅰ、Ⅱ级、无淋巴血管侵犯患者(P<0.05)。结论三阴性乳腺癌患者癌组织中存在PD-1、PD-L1、调节性T淋巴细胞FOXP3的高表达,且其表达水平与高组织学级别、转移密切相关。

Objective To investigate the expression and clinical significance of programmed death molecule-1(PD-1),programmed death molecule ligand-1(PD-L1)and forkhead/winged helix transcription factor(FOXP3)in tissues of patients with triple-negative breast cancer.Methods A total of 70 patients of triple-negative breast cancer and 30 patients of non-triple-negative breast cancer diagnosed in our center from January 2015 to January 2020 were selected as the research objects.The expressions of PD-1,PD-L1 and FOXP3 in the three groups were determined by immunohistochemical method.The differential expressions of PD-1,PD-L1 and FOXP3 in triple-negative breast cancer and non-triple-negative breast cancer tissues and their correlation with the clinical characteristics of patients were analyzed.Results The positive expression rates of PD-1,PD-L1,regulatory T lymphocyte FOXP3 in triple-negative breast cancer were higher than those in non-triple-negative breast cancer(P<0.05).The positive expression rates of PD-1 and PD-L1 in triple-negative breast cancer tissues were not significantly correlated with patient age,tumor diameter(P>0.05).The positive expression rates of PD-1 and PD-L1 in triple-negative breast cancer tissues of patients with gradeⅢ,lymphatic vessel invasion and lymph node metastasis were higher than those in patients with gradeⅠ,gradeⅡ,without lymphatic vessel invasion and without lymph node metastasis(P<0.05).There was no significant correlation between FOXP3 positive expression rate in triple-negative breast cancer tissue and patient age,tumor diameter,lymph node metastasis(P>0.05).The FOXP3 positive expression rates of regulatory T lymphocytes in triple-negative breast cancer tissues of patients with gradeⅢand lymphatic vessel invasion were higher than those in patients with gradeⅠ,gradeⅡand without lymphatic vessel invasion(P<0.05).Conclusion PD-1,PD-L1 and FOXP3 are highly expressed in triple negative breast cancer tissues,and their expression levels are closely related to high histological grade and metastasis.