高倍率大视场细胞内镜成像系统研究

Endocytoscopic Imaging System with High Magnification and Large Field of View

细胞内镜是一种具有超高放大倍率的内窥镜,能够在体内直接观察到染色的细胞核,对早期癌症的诊断具有重要意义,目前仅日本奥林巴斯公司拥有相应的技术。细胞内镜的核心是其头端部的光学成像系统,现有的细胞内镜成像系统在高倍率成像时视场范围较小,检查效率低。本文提出了一种高倍率大视场细胞内镜成像系统,采用6片球面系统实现了高性能低成本的大视场变焦内窥物镜,并基于此搭建了细胞内镜成像系统。实验结果表明:该系统的物方分辨率达到了181.0 lp/mm,放大倍数约为500倍,显微成像视场范围显著增大至1200μm×670μm,是现有细胞内镜成像系统的2倍以上。在此基础上,采用深度学习方法为系统增添了细胞核分割功能,分割准确度达到了96.58%,验证了所搭建的成像系统对细胞核形态细节的成像能力。

Endocytoscopy(EC),as a kind of endoscope with ultra-high magnification,enables the in vivo observation of stained nuclei directly,which is of great significance for the diagnosis of early-stage cancer.Only Olympus of Japan has the technology so far.The core component of EC is the optical imaging system at the tip,and the current endocytoscopic imaging system has a small field of view(FOV)during imaging at high magnification,which leads to low efficiency.In this study,an endocytoscopic imaging system with high magnification and a large FOV has been proposed.We use six-piece spherical system to realize a zoom endoscopic objective lens with a large FOV,which has merits of high performance and low cost.Then,we construct the endocytoscopic imaging system based on this lens.The experimental results show that the object resolution of our system arrives at 181.0 lp/mm,and the magnification is about 500×.In addition,the FOV of microscopic imaging is significantly increased to 1200μm×670μm,which is more than double that of the current endocytoscopic imaging system.On this basis,the nuclear segmentation function is added to the system by deep learning,and the segmentation accuracy reaches 96.58%,which verifies the imaging ability of our system for the details of nuclear morphology.