水稻分蘖期叶片淀粉和蔗糖代谢途径的转录组动态分析

Dynamic Transcriptome Analysis of Starch and Sucrose Metabolism Pathway in Rice Leaves at Tillering Stage

为了探索水稻分蘖期叶片中生育进程相关代谢途径的转录响应情况,分别在分蘖期7 d、14 d和21 d收获水稻品种‘五优稻4号’(‘WYD4’)叶片,并进行动态转录组分析。结果表明,在水稻分蘖期共有12815个差异表达基因,通过GO分析显示这些基因主要集中在细胞组分方面,KEGG分析表明淀粉与蔗糖代谢途径在整个分蘖期生育进程内有差异表达基因(DEGs)显著富集,并有6个淀粉与蔗糖代谢途径相关的差异表达基因在整个分蘖期显著差异表达。同时,预测的转录因子SSⅠ(LOC_Os06g06560)在分蘖前期差异表达倍数达到9.7倍,并与64个预测的蛋白互作。这表明淀粉与蔗糖途径关键基因在不同时间点的应答是有差异的,为水稻分蘖形成和群体构建提供了时间上的能量来源,也为水稻栽培精确调控提供了转录组学指导。

To explore the transcriptional response of metabolic pathways related to growth process,the fresh leaves of rice variety‘Wuyoudao 4’(‘WYD 4’)were harvested at 7 d,14 d and 21 d at the tillering stage,respectively,and immediately frozen with-80℃liquid nitrogen for the dynamic transcriptome analysis.12815 differentially expressed genes(DEGs)were identified,and GO analysis results showed that the DEGs mainly participated in cell components,and KEGG analysis results indicated that throughout the whole tillering stage,the significant DEGs were enriched in starch and sucrose metabolism pathway,and six DEGs in this pathway were identified.Moreover,the expression level of predicted transcription factor SSⅠ(LOC_Os06g06560)was 9.7 in the early tillering stage,and interacted with 64 predicted proteins during the SSⅠanalysis.The results showed that the key genes of starch and sucrose pathway exhibit significantly different expression level at the different stages of rice tillering,which provide the temporal energy for tiller development and population formation.All results can provide transcriptic guidance for precise regulation of rice cultivation.