微滴式数字PCR和实时荧光定量PCR检测大豆中的转基因成分

Detection of Genetically Modified Components in Soybean Products Based on Real-time Fluorescence Quantitative PCR and Digital PCR

本文通过微滴式数字PCR和实时荧光定量PCR,对日常大豆样品进行转基因项目检测,旨在比较2种方法的优缺点,为转基因成分检测探索更优检测方案。本文对转基因基因启动子CaMV35S、终止子NOS基因、大豆内源基因Lectin及GTS 40-3-2品系特异性基因进行比较研究,结果显示,10份市售大豆样品均为转基因成分阴性,微滴式数字PCR及实时荧光定量PCR都可以得出准确结果,可见微滴式数字PCR可以与实时荧光定量PCR配合使用达到优化检验成本的目的。

The purpose of this paper is to compare the advantages and disadvantages of the two methods and explore a better detection scheme for the detection of transgenic components.In this paper,the transgenic gene promoter camv35s,terminator NOS gene,soybean endogenous gene lectin and GTS 40-3-2 strain specific genes were compared.The results showed that 10 commercial soybean samples were negative for transgenic components,and accurate results could be obtained by micro drop digital PCR and real-time fluorescence quantitative PCR,It can be seen that micro drop digital PCR can be combined with real-time fluorescence quantitative PCR to optimize the test cost.