基于微型DNA条形码的黄粉蝶亚科的物种鉴定

Identification of Colia dinae Species Based on the Minimalist-barcode

选用黄粉蝶亚科6属38种38个样本为实验材料,采用虚拟实验(Silico analysis),从BOLD数据库中取得36个样本的序列,同时做实体实验(Empirical test),得到剩余2个样本的COI序列,将所有样本的序列分别拆成648bp、200bp、50bp 3个片段,运用MEGA7.0软件构建NJ树(Neighbor-Joining,Saitou&Nei,1987),并计算出变异位点、简约位点、种内差异和种间差异;对DNA条形码(DNA Barcode)与微型DNA条形码(Minimalist-barcode)进行比较,找出各自的优缺点并探求微型DNA条形码对黄粉蝶亚科鉴定的可行性。结果表明,在鉴定黄粉蝶亚科的准确性方面,微型DNA条形码与DNA条形码基本相同,均可作为黄粉蝶亚科物种鉴定的有效依据。

Thirty-eight samples of the Colia dinae were taken as our experiment objects.We did Silico analysis,acquired 36 samples’COI sequences from BOLD.And we did Empirical test to acquire the other 2 COI sequences.Then,we divided all COI sequences from BOLD into 3 sized segments(648bp,200bp,50bp),and carried out a Neighbor-Joining.We calculated the Variability,Parsimony,Intraspecific and Interspecific.Compared DNA Barcode with Minimalist-barcode to find its advantages and disadvantages and Minimalist feasibility.It turned out the accuracy of Minimalist-barcode in idebtifying species is almost the same as that of DNA Barcode,and both of them can be used as useful evidence for identification of Colia dinae species.