氧化铝改性硅胶复合材料在饮料中诱惑红检测中的应用

Application of alumina modified silica gel composite in the detection of temptation red in beverages

饮料样品中的基体效应复杂,直接检测诱惑红色素是非常困难的。为了提高诱惑红色素检测的灵敏度,有必要对饮料样品进行预处理。将氧化铝修饰硅胶复合材料作为吸附剂,与紫外-可见分光光度计联用,检测饮料样品中的诱惑红色素。实验测得诱惑红色素的最大吸收波长为505 nm,优化了影响固相萃取效率的操作条件,包括吸附剂用量、样品pH、样品体积、洗脱液体积。结果表明,当固相萃取条件为:吸附剂用量为0.18 g,样品pH为2,样品体积为2 mL,洗脱剂用1.5 mL 3%的V(氨水):V(甲醇)=100∶50时,萃取效率最高。在最佳的实验条件下,测得诱惑红的检出限是0.047μg mL^(-1),日内精密度为3.67%,日间精密度为5.39%,加标回收率的范围为89.8%~108.3%。

The matrix effect in beverage samples is complex, and it is very difficult to detect temptation red pigment directly. In order to improve the sensitivity of temptation red pigment detection, it is necessary to pretreat beverage samples. Alumina modified silica gel composite was used as adsorbent and combined with ultraviolet visible spectrophotometer to detect the temptation red pigment in beverage samples. The maximum absorption wavelength of temptation red pigment was 505 nm. The operating conditions affecting the efficiency of solid-phase extraction were optimized, including the amount of adsorbent, sample pH, sample volume and eluent volume. The results showed that the extraction efficiency was the highest when the amount of adsorbent was 0.18 g, the sample pH was 2, the sample volume was 2 mL, and the eluent was 1.5 mL of 3%(V):(V) 100 ∶ 50. Under the best experimental conditions, the detection limit of temptation red was 0.047 μ G mL^(-1), the intra day precision is 3.67%, the intra day precision is 5.39%, and the recovery range of standard addition is 89.8% ~ 108.3%.