基于石墨烯纳米材料对异槲皮苷的电化学检测

Electrochemical detection of isoquercetin based on graphene nanomaterials

以聚乙烯吡咯烷酮(PVP)为保护剂,用水热还原法合成了PVP功能化的还原氧化石墨烯纳米材料(PVP-Gr),用循环伏安法(CV)对PVP-Gr进行了表征。并用PVP-Gr构建电化学传感平台,实现了对异槲皮苷的电化学检测。在优化实验条件的基础上,差分脉冲伏安(DPV)峰电流与浓度为1.0×10^(-10)mol·L^(-1)~5.0×10^(-7)mol·L^(-1)的异槲皮苷呈良好线性关系,检出限为8.0×10^(-11)mol·L^(-1)。将该分析方法应用于药物制剂中异槲皮苷的含量检测,具有重要意义。

PVP functionalized reduced graphene oxide nanomaterials(PVP-Gr) were synthesized by hydrothermal reduction with polyvinylpyrrolidone(PVP) as protective agent. PVP-Gr was characterized by cyclic voltammetry(CV). The electrochemical sensing platform was constructed with PVP-Gr to realize the electrochemical detection of isoquercetin. On the basis of optimizing the experimental conditions, the peak current and concentration of differential pulse voltammetry(DPV) were 1.0 × 10^(-10) mol·L^(-1)~5.0 × 10^(-7) mol·L^(-1) isoquercetin showed a good linear relationship, and the detection limit was 8.0 × 10^(-11) mol·L^(-1). It is of great significance to apply this analytical method to the determination of isoquercetin in pharmaceutical preparations.