lncRNA CBR3-AS1通过调控miR-145-5p影响胃癌HGC-27细胞的增殖、迁移、侵袭和凋亡

lncRNA CBR3-AS1 Affects the Proliferation,Migration,Invasion and Apoptosis of Gastric Cancer HGC-27 Cells by Regulating miR-145-5p

为探讨lncRNA CBR3-AS1靶向miR-145-5p对胃癌细胞HGC-27恶性生物学行为的影响,该研究先用实时荧光定量PCR(RT-qPCR)检测30例胃癌患者的癌组织及癌旁组织中CBR3-AS1和miR-145-5p的表达水平。然后,将CBR3-AS1干扰表达载体质粒、miR-145-5p模拟物、miR-145-5p抑制剂与CBR3-AS1干扰表达载体质粒转染至胃癌细胞HGC-27;用四甲基偶氮唑盐比色法(MTT)检测细胞活性;流式细胞术检测细胞凋亡流程;Transwell法检测迁移和侵袭的细胞数;双荧光素酶报告实验检测CBR3-AS1和miR-145-5p的靶向关系。实验结果显示,胃癌组织中CBR3-AS1表达水平高于癌旁组织,而miR-145-5p表达水平低于癌旁组织(P<0.05)。过表达miR-145-5p或抑制lncRNA CBR3-AS1表达可降低HGC-27细胞活性,减少迁移侵袭细胞数,而提高细胞凋亡率(P<0.05)。lncRNA CBR3-AS1靶向调控miR-145-5p;下调miR-145-5p逆转了抑制lncRNA CBR3-AS1表达对HGC-27细胞的作用。因此,抑制lncRNA CBR3-AS1表达可能通过上调miR-145-5p抑制HGC-27细胞的迁移侵袭、增殖,促进细胞凋亡。

In order to explore the effect of lncRNA CBR3-AS1 targeting miR-145-5p on the malignant biological behavior of gastric cancer HGC-27 cells,RT-qPCR (real-time fluorescent quantitative PCR) was performed to examine the expression levels of CBR3-AS1 and miR-145-5p in cancerous tissues and adjacent tumors of 30 patients with gastric cancer.Then,the CBR3-AS1 interference expression vector plasmid,miR-145-5p mimic,miR-145-5p inhibitor and CBR3-AS1 interference expression vector plasmid were transfected into gastric cancer cell HGC-27;cell viability was detected by MTT (tetramethylazolium salt colorimetry);flow cytometry was used to detect cell apoptosis rate;Transwell method was used to detect the cells number of migration and invasion;dual luciferase report test was used to detect the targeting relationship between CBR3-AS1 and miR-145-5p.Experiment result showed that the expression level of CBR3-AS1 in gastric cancer tissue was higher than that in adjacent tissues,while the expression level of miR-145-5p was lower than that in adjacent tissues (P<0.05).Overexpression of miR-145-5p or inhibition of lncRNA CBR3-AS1 expression can reduce the activity of HGC-27 cells,the number of migration and invasion cells,and increase the apoptosis rate (P<0.05).lncRNA CBR3-AS1 targeted and regulated miR-145-5p.Down-regulation of miR-145-5p reversed the effect of inhibiting the expression of lncRNA CBR3-AS1 on HGC-27 cells.Therefore,inhibiting the expression of lncRNA CBR3-AS1 may inhibit the migration,invasion and proliferation of HGC-27 cells,and promote cell apoptosis by up-regulating miR-145-5p.