环状RNA hsa_circ_0079557在胃癌中的表达及其临床意义

Expression of circular RNA hsa_circ_0079557 in gastric cancer and its clinical significance

目的:探讨hsa_circ_0079557在胃癌中的表达水平和临床价值。方法:收集5对胃癌组织及癌旁组织,高通量测序技术检测后结合circbase等数据库中胃癌数据分析,锁定差异表达的部分环状RNA。用qRT-PCR检测62对胃癌标本中hsa_circ_0079557表达,分析其与临床病理特征的关系;检测并分析hsa_circ_0079557在胃癌细胞(SGC-7901、BGC-823、HGC-27和MGC-803)和胃黏膜上皮GES-1细胞、胃癌患者和健康体检者血清中表达差异。siRNA敲减hsa_circ_0079557后,用细胞克隆形成实验、平板划痕实验、细胞周期流式实验分别检测胃癌细胞(HGC-27和MGC-803)增殖和迁移能力及细胞周期改变,蛋白印迹测定细胞周期蛋白依赖激酶3(cyclin dependent kinase 3,CDK3)表达水平。结果:qRT-PCR结果显示,hsa_circ_0079557在胃癌组织中表达水平明显高于癌旁组织,且其表达水平与肿瘤TMN分期相关(P均<0.05);hsa_circ_0079557在胃癌细胞中表达水平明显高于胃黏膜上皮细胞(P<0.05);hsa_circ_0079557在胃癌患者血清中水平明显高于健康体检者(P<0.05)且术前明显高于术后(P<0.01);hsa_circ_0079557敲减后HGC-27和MGC-803细胞增殖、MGC-803细胞迁移能力下降,HGC-27和MGC-803细胞CDK3表达水平下降(P<0.05)。胃癌细胞株中hsa_circ_0079557敲减使细胞多阻滞于G0-G1期。结论:hsa_circ_0079557在胃癌组织中呈高表达,其可作为胃癌潜在的早期诊断及预后生物标志物。

Objective:To investigate the expression level and clinical value of hsa_circ_0079557 in gastric cancer.Methods:Five pairs of gastric cancer tissues and adjacent tissues were collected and detected by high-throughput sequencing technology,combined with the data of gastric cancer in circbase and other databases,to screen for differentially expressed circRNAs.In 62 pairs of samples,hsa_circ_0079557 was detected by qRT-PCR,and its relationship with clinicopathological characteristics was analyzed.The expression levels of hsa_circ_0079557 in gastric cancer cells and gastric mucosal epithelial cells,preoperative and postoperative serums of gastric cancer patients,and serums of gastric cancer patients and healthy people were detected.After hsa_circ_0079557 was knocked down by siRNA,cell clone formation assay,plate scratch assay,and cell cycle flow cytometry assay were used to detect the changes in cell cloning,migration and cell cycle of gastric cancer cells,respectively.Western blotting was used to determine cyclin dependent kinase 3(CDK3)expression level.Results:The expression level of hsa_circ_0079557 in human gastric cancer tissues was significantly higher than that in adjacent tissues(P<0.05),and its expression level was related to the tumor TMN stage(P<0.05);the expression level of hsa_circ_0079557 in gastric cancer cells was significantly higher than that in human gastric mucosal epithelial cells(P<0.05);the expression level of hsa_circ_0079557 in serum of gastric cancer patients before operation was significantly higher than that after operation(P<0.01);hsa_circ_0079557 knockdown in gastric cancer cell lines resulted in decreased proliferation and migration ability of some gastric cancer cells and decreased CDK3 expression level(P<0.05).In gastric cancer cell lines,hsa_circ_0079557 knockdown resulted in cell arrest in G0-G1 phase.Conclusion:Hsa_circ_0079557 is highly expressed in gastric cancer tissues,which might be used as a potential biomarker for early diagnosis and prognosis of gastric cancer.