HPLC法测定和肝利胆颗粒中黄芩苷和黄芩素的含量

Determination of Baicalin and Baicalein in Heganlidan Granules by HPLC

目的:建立高效液相色谱法测定和肝利胆颗粒中黄芩苷和黄芩素含量的方法。方法:采用Agilent 5TC-C_(18)(2)色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈(A)-0.1磷酸溶液(B)(梯度洗脱:0~20min:23%A,20~25 min:23%→33%A,25~40 min:33%A,40~45 min:33%→23%A,45~50 min:23%A),流速1.0 m L/min,检测波长283 nm,柱温30.0℃。结果:黄芩苷和黄芩素线性范围分别为144.4~1444 ng,r=0.9998;102.0~1020 ng,r=0.9999。平均回收率分别为98.67%和99.54%,RSD分别为0.79%和1.01%。测定3批样品,结果表明黄芩苷和黄芩素的含量分别在3.72~4.77 mg/g和0.69~1.16 mg/g之间。该方法操作简便、重复性好、结果准确,可作为和肝利胆颗粒质量的控制方法。

To establish an HPLC method for the simultaneous determination of Baicalin and Baicalein in Heganlidan Granules. Agilent 5 TC-C_(18)( 2) column( 250 mm × 4. 6 mm,4 μm) was used,the mobile phase was acetonitrile( A)-0. 1% phosphoric acid aqueous solution( B)with gradient elution( 0 ~ 20 min,23% A;20 ~ 25 min,23% →33% A;25 ~ 40 min,33% A;40 ~ 45 min,33% →23% A;45 ~ 50 min;23% A).The flow rate was 1. 0 m L·min^(-1),the detection wavelength was 270 nm,and the column temperature was 30. 0 ℃ . The linear ranges of Baicalin and Baicalein fell into 144. 4 ~ 144 4 ng( r = 0. 999 8) and 102. 0 ~ 102 0 ng( r = 0. 999 9) respectively,and the average recovery were98. 67% and 99. 54% with RSD as 0. 79% and 1. 01%,respectively. The test results of 3 batches of samples showed that the contents of Baicalin and Baicalein fell into 3. 72 ~ 4. 77 mg·g^(-1) and 0. 69 ~ 1. 16 mg·g^(-1) respectively. The established method has the simple operation,good repeatability and accuracy. It can be used for quality control of Heganlidan Granules.