抵抗素对雄性小鼠生殖功能的影响

Effects of Resistin on Reproductive Function of Male Mice

以C57BL/6J雄性小鼠为试验对象,利用抵抗素重组蛋白进行尾静脉注射,模拟高循环水平抵抗素状态,探讨抵抗素对雄性小鼠生殖功能的影响。10只8周龄无特定病原体(SPF)级C57BL/6J雄性小鼠随机分为试验组和对照组,试验组小鼠注射抵抗素400 ng/(d•只),对照组小鼠注射等量的双蒸水。7 d后测定小鼠血清生殖激素水平的变化,检查精子的活动能力以及睾丸组织结构和形态变化。研究结果发现:试验组小鼠的血清雌二醇(E2)质量浓度和睾酮(T)质量浓度均显著高于对照组小鼠(P<0.05),促卵泡成熟素(FSH)质量浓度和促黄体生成素(LH)质量浓度与对照组差异不显著(P>0.05)。试验组小鼠精子活力显著下降,精子形态异常现象显著增加。试验组小鼠曲精小管直径小于对照组,管腔相对较大,睾丸出现超微结构病变。抵抗素能够引起小鼠血清性激素水平变化,导致小鼠生殖功能下降。

In this paper,C57BL/6J male mice were injected with resistin recombinant protein via tail vein to simulate high circulating resistin level and to explore the effects of resistin on reproductive function of male mice.Ten 8-week-old specific pathogen free(SPF)C57BL/6J male mice were randomly divided into experimental group and control group.The each mice in the experimental group were injected with resistin 400 ng/d,while the mice in the control group were injected with the same amount of double distilled water.Seven days later,the changes of serum hormone levels,sperm motility,testicular structure and morphology were detected.The results show that the mass concentration of serum estradiol(E2)and testosterone(T)in experimental group were significantly higher than those in control group(P<0.05).There was no significant difference in the mass concentration serum follicle stimulating hormone(FSH)and luteinizing hormone(LH)in experimental group with control group(P>0.05).In the experimental group,sperm motility decreased significantly and sperm morphological abnormality increased significantly.The diameter of seminiferous tubules in experimental group is smaller than that in control group,the lumen is relatively larger,and testicular ultrastructural lesions appeare.Resistin causes the change of serum sex hormone level and decreases the reproductive function of mice.