多功能脱氧核酶用于生物医学分析的研究进展

Advances of Multifunctional Deoxyribozyme in Biomedical Analysis

脱氧核酶(DNAzymes)是一类人工合成的通过指数富集式配体系统进化技术(SELEX)筛选得到的具有催化功能的单链DNA分子.由于DNAzyme具有易于合成和修饰、化学结构稳定及催化活性高等优点,近年来在生物传感和医学诊断领域备受关注.对DNAzyme的活性进行调控是挖掘其多方面应用潜能的关键,灵活的活性调控方式将促进DNAzyme在不同领域的应用.本文综合评述了一些调控脱氧核酶活性的主要方法,并对其在生物医学分析领域方面的应用进行了简要介绍.

Deoxyribozymes(DNAzymes)are synthetic deoxynucleotide oligomers with enzyme-like activities identified through in vitro SELEX selection technology.DNAzymes have been widely explored in a broad range of applications,notably in biosensing and biomedical devices,owing to their easy synthesis and modification,stable chemical structure and excellent catalytic activity.Regulating the activity of DNAzymes is the key for exploring its potential utilization in many fields,and the flexible control will dramatically promote the application of DNAzymes.In this review,we summarize some methods for the regulation of DNAzymes activity and introduce their main applications in biomedical analysis.It is believed that DNAzymes will play a critical role in chemistry and medicine in the future.