摘要
目的:基于代谢组学方法研究慢性阻塞性肺病(chronic obstructive pulmonary disease, COPD)稳定期大鼠支气管肺泡灌洗液(bronchoalveolar lavage fluid, BALF)的潜在生物标志物。方法:采用烟熏联合细菌反复感染的方法制备COPD稳定期模型大鼠。采用超高效液相色谱-串联质谱技术采集大鼠的BALF数据,利用多元统计分析软件筛选差异代谢物,再结合数据库鉴定潜在生物标志物,并进行代谢通路分析。结果:多元统计分析结果显示,正常组与模型组BALF代谢物发生显著变化。通过比对数据库,共鉴定出30个差异性代谢物,主要涉及到体内的氨酰基-tRNA的生物合成,甘氨酸、丝氨酸和苏氨酸的代谢,鞘脂代谢,苯丙氨酸代谢和花生四烯酸代谢等8条代谢通路。结论:基于代谢组学研究COPD稳定期的BALF代谢物变化,寻找COPD稳定期相关代谢标志物,为治疗COPD提供理论参考。
OBJECTIVE To explore the potential biomarkers of bronchoalveolar lavage fluid(BALF) in rats with stable chronic obstructive pulmonary disease(COPD) based upon metabonomics.METHODS A rat stable COPD model was established by recurrent bacterial infections plus smoking. Ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) was employed for collecting the data of lavage fluid. Multivariate statistical analytic software was utilized for metabolite screening. And the database was accessed for identifying potential biomarkers and metabolic pathways.RESULTS Multivariate statistics revealed a significant change of BALF metabolite between control and model groups. Through a database comparison, a total of 30 different metabolites were correlated with the in vivo biosynthesis of aminoacyl-tRNA and the metabolisms of glycine, serine, threonine, sphingolipid, phenylalanine, arachidonic acid and enoic acid.CONCLUSION Based upon the studies of metabolite changes in BALF of stable COPD metabolomics, stable COPD-related metabolic markers provide theoretical references for the treatment of lung diseases.
作者
吕田田
余亚辉
余海艳
薛丙权
李康
黄海英
LV Tian-tian;YU Ya-hui;YU Hai-yan;XUE Bing-quan;LI kang;HUANG Hai-ying(Department of Pharmacy,Henan University of Traditional Chinese Medicine,Henan Zhengzhou 450046,China)
出处
《中国医院药学杂志》
CAS
北大核心
2021年第19期1955-1961,共7页
Chinese Journal of Hospital Pharmacy
基金
国家自然科学基金(编号:81904021)
河南省2019年骨干教师项目(编号:2019GGJS109)
河南中医药大学研究生科研创新项目(编号:2020KYCX023)。
关键词
代谢组学
超高效液相色谱-串联质谱法
慢性阻塞性肺病
支气管肺泡灌洗液
生物标志物
metabolomics
ultra performance liquid chromatography-tandem mass spectrometry
chronic obstructive pulmonary disease
bronchoalveolar lavage fluid
biomarker