人参皂苷Rh3抑制卵巢癌细胞增殖、迁移、侵袭机制研究

Mechanism of ginsenoside Rh3 inhibiting proliferation,migration and invasion of ovarian cancer cells

目的研究人参皂苷Rh3对卵巢癌细胞增殖、迁移、侵袭的影响。方法采用人参皂苷Rh3按浓度及时间梯度处理卵巢癌SKOV3细胞,对照组为含同浓度溶剂的无药物处理,实验组人参皂苷Rh3的浓度分别为50、75、150、300μg/mL,使用CCK8法测定及平板克隆实验测定不同浓度人参皂苷Rh3对SKOV3细胞增殖的调控作用;选取最适浓度及最佳作用时间进行后续实验,利用细胞划痕实验检测不同浓度人参皂苷Rh3在不同处理时间后对SKOV3细胞迁移的影响;使用Transwell侵袭实验检测不同浓度人参皂苷Rh3在不同时间段对SKOV3细胞侵袭的影响。结果 CCK8结果表明,75μg/mL及150μg/mL的人参皂苷Rh3处理人卵巢癌SKOV3细胞6 h、12 h后,SKOV3细胞活力被显著抑制(P<0.05),且呈浓度和时间依赖性;同时平板克隆实验显示,细胞增殖能力随药物浓度增加显著下降(P<0.05)。而与对照组比较,在相同作用时间下,低浓度的人参皂苷Rh3(50μg/mL)对SKOV3细胞增殖的影响差异不显著(P>0.05);划痕实验显示,75μg/mL及150μg/mL的人参皂苷Rh3对人卵巢癌SKOV3细胞作用24 h、36 h均可显著抑制SKOV3细胞划痕的愈合(P<0.05),且Transwell细胞侵袭实验也表明人参皂苷Rh3处理组穿过微孔膜的细胞数目较对照组明显减少(P<0.05)。结论人参皂苷Rh3能够显著抑制人卵巢癌SKOV3细胞活力及其增殖、迁移、侵袭能力。

Objective To study the effects of ginsenoside Rh3 on proliferation, migration and invasion of ovarian cancer cells. Methods SKOV3 cells were treated with ginsenoside Rh3 in a concentration and time dependent manner. The control group was treated with the same concentration of solvent without drugs. The concentrations of ginsenoside Rh3 in experimental groups were 50, 75, 150, and 300 μg/mL, respectively. Cell Counting Kit-8 assay(CCK8) and plate cloning asssy were performed to detect the effect of ginsenoside Rh3 in different concentrations on cell proliferation of SKOV3 cells. Optimal concentration was determined for subsequent experiments. Cell migration and invasion was measured by cell scratch assay and transwell invasion assay with different concentrations of ginsenoside Rh3 on SKOV3 cells. Results CCK8 assays showed that treating SKOV3 cells with 75 μg/mL and 150 μg/mL ginsenoside Rh3 for 6 h and 12 h significantly inhibited cell viability in a concentration and time-dependent manner(P<0.05), meanwhile, plate cloning asssy showed that cell proliferative ability was significantly decreased in a dose-dependent manner(P<0.05). Compared with the control group, there was no significant difference on cell proliferation in low concentration of ginsenoside Rh3(50 μg/mL). In cell scratch tests, 75 μg/mL and 150 μg/mL ginsenoside Rh3 suppressed the wound healing efficacy significantly by treatment of 24 h and 36 h separately(P<0.05). Simultaneously, transwell invasion assay showed that the number of SKOV3 cells passing through the microporous membrane with ginsenoside Rh3 treatment group was significantly lower than that in the control group(P<0.05). Conclusion Ginsenoside Rh3 can significantly inhibit the cell viability, proliferation, migration and invasion of human ovarian cancer SKOV3 cells.