桃儿七质量标准的研究

Quality Standard for Sinopodophyllum hexandrum

目的建立桃儿七Sinopodophyllum hexandrum(Royle)Ying的质量标准。方法分类学方法鉴别桃儿七基源性状,显微鉴别和薄层色谱法(TLC)鉴别桃儿七基源,测定杂质、水分、总灰分、酸不溶性灰分、重金属及有害元素、农药残留、醇溶性浸出物含量,HPLC法测定4′-去甲基鬼臼毒素、槲皮素、山柰酚、鬼臼毒素含量。结果桃儿七显微鉴别与薄层鉴别特征显著。初步拟定桃儿七中杂质不得超过4.00%,水分不得超过9.00%,总灰分含量不得超过4.00%,酸不溶性灰分含量不得超过1.00%,铅含量不得超过5 mg/kg,镉含量不得超过1 mg/kg,砷含量不得超过2 mg/kg,汞含量不得超过0.2 mg/kg,铜含量不得过20 mg/kg,马拉硫磷、杀螟硫磷、抗蚜威、溴氰菊酯、甲氰菊酯含量均不得超过0.01 mg/kg,醇溶性浸出物含量不得少于5.00%,鬼臼毒素含量不得少于2.80 mg/g。结论该方法简便,专属性强,重复性好,可有效控制桃儿七的质量。

AIM To establish the quality standard for Sinopodophyllum hexandrum(Royle)Ying.METHODS S. hexandrum was subjected to the taxonomic identification, the detection of morphologic features by microscopy, and quantitative determinations of impurities moisture, total ash, acid insoluble ash, heavy metals and harmful elements, pesticide residues, and alcohol-soluble extract were determined by TLC;and the content determination of 4′-demethylpodophyltoxin, quercetin, kaempferol, podophyllotoxin were determined by HPLC.RESULTS The microscopic identification and TLC identification contributed significant distinguishing characteristics for S. hexandrum. It was preliminarily proposed that there should be no more than 4.00% impurities, 9.00% moisture, 4.00% total ash, 1.00% acid insoluble ash, 5 mg/kg Pb, 1 mg/kg Cd, 2 mg/kg As, 0.2 mg/kg Hg, 20 mg/kg Cu, 0.01 mg/kg malathion, sumithion, pirimicarb, deltamethrin, and fenpropathrin;no less than 5.00% alcohol-soluble extract and 2.80 mg/g podophyllotoxin.CONCLUSION This simple, specific and reproducible method can be used for the effective quality control of S. hexandrum.