干扰FGF4表达可抑制口腔鳞状细胞癌细胞增殖、迁移及侵袭能力

Down regulation of fibroblast growth factor 4 lowers the viability and inhibits the migration and invasion of oral squamous cell carcinoma cells

目的探索干扰成纤维细胞生长因子4(fihmhlast growth factor 4,FGF4)表达对口腔鱗状细胞癌(oral squamous fell rarrinoma,0SCC)细胞增殖、迁移及侵袭能力的影响_,方法培养5株0SCC细胞株并检测FGF4 DMA拷贝数及mRNA的表达变化;转染siRNA干扰FGF4表达并进行验证;干扰FGF4表达后,采用CCK-8检测0SCC细胞株HSC-3、H3I4细胞增殖能力的变化,采用Transwell方法检测细胞迁移及侵袭能力的变化。结果同对照组相比,干扰FGF4表达后,HSC-3、H314细胞增殖能力明显下降(P<0.05)。在HSC-3中,FGF4 siRNA-002组及siRNA-003组迁移细胞数分别下降了64.52%、66.81%;在H314细胞系中,FGF4siRNA-002组及siRNA-003组迁移细胞数下降了66.81%、82.01%。同对照组相比,在HSC-3中,FGF4siRNA-002组及siRNA-003侵袭细胞数分别下降了52.34%、49.89%。在H314中,FGF4 siRNA-002组及siRNA-003侵袭细胞数分别下降了75.3%、90.63%。结论在0SCC细胞中,干扰FGF4表达可抑制0SCC细胞的增殖、迁移及侵袭能力.

Objective To investigate the role of FGF4 in regulating cell biological behavior in OSCC. Methods Five different OSCC cell lines were cultured for determining FGF4 DNA copy numbers. HSC-3 and H314 cells were transfected with FGF4-specific siRNA to down-regulate FGF4 expression. The cell viability, migration, and invasion of FGF4-depleted cells were then analyzed and compared with that of control cells. Results Compared with the control group, the cell viability was decreased in FGF4-deficient HSC-3 and H314 cell lines. In HSC-3 cell lines, the number of migrated cells in the FGF4-deficient cells decreased by 64.52%(siRNA-002) and 66.81%(siRNA-003).In H314 cell lines, the number of migrated cells in the FGF4-deficient cells decreased by 66.81%(siRNA-002) and 82.01%(siRNA-003). In HSC-3 cell lines, the number of invasive cells in the FGF4-deficient cells decreased by 52.34%(siRNA-002) and 49.89%(siRNA-003).In H314 cell lines, the number of invasive cells in the FGF4-deficient cells decreased by 75.3%(siRNA-002) and 90.63%(siRNA-003). Conclusions FGF4-deficient OSCC cells exhibited lower cell viability, migration, and invasion than the control cells.