良恶性肾上腺嗜铬细胞瘤VEGF、melanA、LncRNA HOTAIR表达差异及与患者预后的关系

Differential expression of VEGF,melanA and lncRNA HOTAIR in benign and malignant adrenal pheochromocytoma and their relationship with prognosis

目的探讨良恶性肾上腺嗜铬细胞瘤(PCC)组织血管内皮生长因子(VEGF)、黑色素A(melanA)、长链非编码RNA(lncRNA)HOX基因的反义基因间RNA(LncRNA HOTAIR)表达差异及与患者预后的关系。方法选取肥城查庄矿医院肾内科收治的69例恶性PCC为恶性组,33例良性PCC为良性组。采集手术切除的良恶性PCC组织标本,比较两组VEGF、melanA、LncRNA HOTAIR表达,分析上述指标表达与恶性组临床病理特征的相关性,评价上述指标对良恶性肾上腺PCC的鉴别效能及与1年生存率的关系。结果恶性组VEGF、LncRNA HOTAIR阳性表达率高于良性组,melanA阳性表达率低于良性组,差异有统计学意义(P<0.05);VEGF、melanA、LncRNA HOTAIR表达与恶性组临床分期、浸润程度、淋巴结转移、分化程度存在一定相关性(P<0.05);VEGF、melanA、LncRNA HOTAIR联合鉴别恶性肾上腺PCC的曲线下面积(AUC)最大,为0.919(P<0.05)。VEGF、LncRNA HOTAIR阳性表达患者1年生存率低于阴性表达患者,melanA阳性表达患者1年生存率高于阴性表达患者(P<0.05)。结论恶性PCC患者VEGF、LncRNA HOTAIR表达升高,melanA表达下降,三者联合检测可作为PCC良性与恶性鉴别及预后预测的潜在指标。

Objective To investigate the expression of vascular endothelial growth factor(VEGF),melanin A(melanA)and long non-coding RNA(lncRNA)HOX antisense intergenic RNA(HOTAIR)in benign and malignant adrenal pheochromocytoma(PCC)and its relationship with prognosis.Methods 69 cases of malignant PCC were selected as the malignant group and 33 cases of benign PCC as the benign group.The expressions of VEGF,MelanA and lncRNA HOTIA in benign and malignant PCC were compared.The correlation between the expression of VEGF,MelanA and lncRNA HOTAIR and the clinicopathological features of the malignant group was analyzed.The differential efficacy of the above indicators for benign and malignant adrenal PCC and their relationship with 1-year survival rate were evaluated.Results the positive expression rate of VEGF and lncRNA HOTAIR in the malignant group was higher thanthat in the benign group,and the positive expression rate of Melan A was lower than that in the benign group(P<0.05).The expression of VEGF,Melan A and lncRNA HOTAIR was correlated with the clinical stage,degree of invasion,lymph node metastasis and degree of differentiation in the malignant group(P<0.05).The area under the curve(AUC)of VEGF,melanA,lncRNA HOTAIR combined to identify malignant adrenal PCC is the largest,0.919.The 1-year survival rate of patients with positive expression of VEGF and lncRNA HOTAIR was lower than that of patients with negative expression,and the 1-year survival rate of patients with positive expression of Melan A was higher than that of patients with negative expression(P<0.05).Conclusion The expression of VEGF and lncRNA HOTAIR in patients with malignant PCC is increased,and the expression of melanA is decreased.The combined detection of the three can be used as a potential indicator for the differentiation of benign and malignant PCC and prognostic prediction.