系统性红斑狼疮患儿中性粒细胞亚群与外周血自噬相关基因Beclin1表达水平相关性

Relationship between peripheral blood neutrophil subsets and expression of autophagy related gene Beclin1 in children with systemic lupus erythematosus

目的探讨系统性红斑狼疮(SLE)患儿中性粒细胞亚群与外周血自噬相关基因Beclin1(Beclin1)的相关性。方法选取2018年01月至2019年12月期间就诊于本院儿科并确诊为SLE的56例患儿为研究对象,纳入为SLE组,根据SLE活动度评分表(SLEDAI)分为活动期(32例)和非活动期(24例);对照组为同期健康体检儿童25例。对比两组之间白细胞、中性粒细胞、淋巴细胞、血红蛋白、抗dsDNA抗体、ANA抗体、抗Sm抗体、APL阳性、补体C3、低密度粒细胞(LDGs)、SLEDAI评分以及外周血Beclin1的表达水平。结果SLE组患儿的LDGs比例、抗dsDNA抗体比例、SLEDAI评分及Beclin1均明显高于对照组,补体C3水平低于对照组,差异均有统计学意义(P<0.05)。活动期SLE患儿的补体C3水平较非活动期患儿低,LDGs比例、抗dsDNA抗体比例、SLEDAI评分及Beclin1均高于非活动期患儿,差异有统计学意义(P<0.05)。Pearson相关分析结果显示,SLE患儿的LDGs比例与SLEDAI评分(r=0.245,P=0.033)及Beclin1(r=0.356,P=0.002)呈正相关,而与补体C3、抗dsDNA抗体则无明显相关性。结论SLE患儿表现为LDGs升高和Beclin1高表达,二者在SLE的发生发展过程中可能存在正向相关作用。

Objective To investigate the relationship between neutrophil subsets and peripheral blood autophagy related gene Beclin1 in children with systemic lupus erythematosus(SLE).Methods 56 children with SLE diagnosed in pediatrics of our hospital from January 2018 to December 2019 were selected as the research objects,and they were divided into the active stage(32 cases)and inactive stage(24 cases)according to SLE activity rating scale(SLEDAI).25 cases of healthy children in the same period were selected as the control group.White blood cells,neutrophils,lymphocytes,hemoglobin,anti dsDNA antibody,ANA antibody,anti SM antibody,APL positive,complement C3,low density granulocyte(LDGs)and SLEDAI scores were measured and compared.The level of Beclin1 in peripheral blood was detected by real-time quantitative RT-qPCR.Pearson correlation was used to analyze the correlation among the parameters.Results The proportion of LDGs,anti dsDNA antibody,SLEDAI score and Beclin1 in the SLE group were significantly higher than those in the control group,while complement C3 level in the SLE group was lower than that in control group(P<0.05).The level of complement C3 in the active SLE patients was lower than that in the inactive SLE patients,while the proportion of LDGs,anti dsDNA antibody,SLEDAI score and Beclin1 in the active SLE patients were higher than that in the inactive SLE patients(P<0.05).The results of pearson correlation analysis showed that the proportion of LDGs in children with SLE was positively correlated with SLEDAI score(r=0.245,P=0.033)and Beclin1(r=0.356,P=0.002),but there was nosignificant correlation with complement C3 and anti ds DNA antibody.Conclusion Children with SLE show increased LDGs and high-level expression of Beclin1.LDGs and Beclin1 may have a positive correlation in the occurrence and development of SLE.