侧蒿中一个新的桉烷型倍半萜及其肿瘤细胞毒活性

Tumour cytotoxic activities of a new eudesmane-type sesquiterpenoid from Artemisia deversa Diels

采用溶剂提取法,Diaion HP-20大孔树脂、CHP20/P120 MCI、Sephadex LH-20葡聚糖凝胶、硅胶及制备高效液相等色谱方法对侧蒿的乙醇提取物进行分离纯化,得到5个化合物,根据质谱、核磁共振谱以及X-ray单晶衍射等数据确定了它们的结构,分别为侧蒿缩醛(1)、7-羟基-6-甲氧基香豆素(2)、6,7-二甲氧基香豆素(3)、咖啡酸(4)和4',5,7-三羟基黄酮(5)。其中,化合物1为新化合物。采用甲基噻唑蓝法(MTT)测试了化合物1~5对四种细胞株(人肝癌细胞HepG2、人非小细胞肺癌细胞A549、人宫颈癌细胞HeLa、人胚肺细胞MRC-5)的体外细胞毒活性,结果表明,化合物1和5对A549和HepG2显示出较强的生长抑制活性,IC_(50)值分别为8.36、16.51μmol·L^(-1)和17.06、7.95μmol·L^(-1)。

Five compounds were isolated from an ethanol extract of Artemisia deversa Diels by solvent extraction,Diaion HP-20,CHP20/P120 MCI,Sephadex LH-20,silica gel and preparative high performance liquid chromatography.Their structures were elucidated by MS,NMR and X-ray as artemideversal(1),7-hydroxy-6-methoxycoumarin(2),6,7-dimethoxycoumarin(3),caffeic acid(4)and 4',5,7-trihydroxy flavone(5).Compound 1 is a new eudesmane-type sesquiterpene.In vitro cytotoxic activities of the five compounds were explored by MTT testing with HepG2,A549,HeLa and MRC-5 cell lines.Results show that compounds 1 and 5 significantly inhibited cellular proliferation.The IC_(50) of compound 1 in A549 and HepG2 cells was 8.36 and 16.51μmol·L^(-1);the IC_(50) of compound 5 in A549 and HepG2 cells was 17.06 and 7.95μmol·L^(-1).