过表达miR-320a对宫颈癌Hela细胞增殖和侵袭能力的影响及其可能机制

The effect of overexpression of miR-320a on proliferation and invasion of cervical cancer Hela cells and its possible mechanism

目的探讨过表达miR-320a对宫颈癌Hela细胞增殖和侵袭能力的影响。方法将宫颈癌Hela细胞用培养基培养后,将miR-320a模拟物瞬时转染Hela细胞使miR-320a过表达,并通过实时定量PCR进行验证,采用CCK-8方法、Transwell实验检测各组Hela细胞的增殖和侵袭能力,Western blot检测各组Hela细胞E2F1蛋白表达,应用Targetscan7.2网站预测E2F1与miR-320a的靶向关系,应用双荧光素酶报告基因实验进行验证。结果在转染miR-320a模拟物后,Hela细胞中miR-320a表达上升,细胞增殖能力和侵袭能力下降,E2F1蛋白表达下降,生物信息学分析及双荧光素酶实验证实E2F1为miR-320a的靶基因。结论过表达miR-320a可以抑制Hela细胞增殖与侵袭,其机制可能与调控E2F1的表达相关。

Objective To investigate the effect and mechanism of overexpression of miR-320 a on proliferation and invasion of Hela cells. Methods Cervical carcinoma cells Hela were cultured, and miR-320 a mimics were transiently transfected into Hela cells to overexpress miR-320 a, and verified by real-time quantitative PCR. CCK-8 and Transwell were used to detect the proliferation and invasion of Hela cells. Western blot was used to detect the expression of E2 F1 protein, and whether E2 F1 was the target gene of miR-320 a was predicted by Targetscan 7.2, which was verified by double luciferase reporter gene experiment. Results In miR-320 a mimics group, the expression of miR-320 a was increased, the expression of E2 F1 was down-regulated, the proliferation and invasion was decreased. Bioinformatics analysis and dual luciferase reporter gene showed that E2 F1 was one of the target genes of miR-320 a. Conclusion The overexpression of miR-320 a could inhibit the proliferation and invasion of Hela, which may be related to the regulation of E2 F1 expression.