PRRX1对唾液腺腺样囊性癌细胞自噬的影响

Effect of PRRX1 on autophagy of salivary adenoid cystic carcinoma cells

目的:探讨配对相关同源框1(paired related homeobox1,PRRX1)对唾液腺腺样囊性癌(salivary adenoid cystic carcinoma,SACC)细胞自噬的影响。方法:用PRRX1过表达慢病毒载体及PRRX1慢病毒空载体转染SACC细胞后,应用Western蛋白免疫印迹法检测转染效果。利用透射电子显微镜观察自噬小体,通过细胞免疫荧光和蛋白免疫印迹法检测自噬标志物(LC3-Ⅱ/Beclin1)水平。采用SPSS 17.0软件包对数据进行统计学分析。结果:与未转染的空白对照组和阴性空病毒载体转染组相比,PRRX1过表达组的PRRX1表达量升高,自噬小体减少,自噬标志物水平降低(P<0.05)。结论:PRRX1对SACC细胞的自噬有抑制作用。

PURPOSE:To investigate the effect of paired related homeobox1(PRRX1)on autophagy of salivary adenoidcystic carcinoma(SACC)cells.METHODS:PRRX1-overexpressed lentiviral vectors and their negative control lentiviral vectors were used to transfect SACC cells.The transfection effect was detected by Western blot.Autophagosome was ob-served under transmission electron microscopy(TEM).The level of autophagy markers(LC3-Ⅱ/Beclin1)was detected by immunofluorescence and Western blot.Statistical analysis was performed with SPSS 17.0 software package.RESULTS:PRRX1 protein level increased,autophagosome number decreased,and autophagy marker level decreased in the PRRX1 overexpressed group,compared to the control(P<0.05).CONCLUSIONS:PRRX1 inhibits the autophagy of SACC cells.