血清miR⁃199a⁃5p、miR⁃378a⁃3p水平与婴幼儿增殖期面部血管瘤普萘洛尔停药后复发的关系

Relationship between serum miR⁃199a⁃5p and miR⁃378a⁃3p levels and recurrence of infants with proliferative facial hemangioma after propranolol withdrawal

目的探讨血清miR⁃199a⁃5p、miR⁃378a⁃3p水平与婴幼儿增殖期面部血管瘤普萘洛尔停药后复发的关系。方法增殖期面部血管瘤患儿93例,均接受普萘洛尔治疗,随访治疗后血管瘤复发情况,并将患儿分为复发组(23例)和无复发组(70例)。治疗前、后采集静脉血,qRT⁃PCR检测血清miR⁃199a⁃5p、miR⁃378a⁃3p表达,分析血清miR⁃199a⁃5p、miR⁃378a⁃3p表达与增殖期面部血管瘤婴幼儿普萘洛尔停药后复发的关系。结果无复发组治疗后血清miR⁃199a⁃5p、miR⁃378a⁃3p表达较治疗前增高(P<0.05),复发组治疗后血清miR⁃199a⁃5p、miR⁃378a⁃3p表达与治疗前比较差异无统计学意义(P>0.05)。复发组治疗后血清miR⁃199a⁃5p、miR⁃378a⁃3p表达低于无复发组(P<0.05)。治疗后瘤体分级Ⅲ~Ⅳ级患儿血清miR⁃199a⁃5p、miR⁃378a⁃3p表达高于Ⅰ~Ⅱ级患儿(P<0.05)。Logistic回归分析结果显示miR⁃199a⁃5p低表达、miR⁃378a⁃3p低表达、治疗后瘤体分级Ⅰ~Ⅱ级是增殖期面部血管瘤婴幼儿普萘洛尔停药后复发的危险因素(P<0.05)。结论血清miR⁃199a⁃5p、miR⁃378a⁃3p低表达与增殖期面部血管瘤婴幼儿普萘洛尔停药后血管瘤复发有关。

Objective To investigate the relationship between serum miR⁃199a⁃5p and miR⁃378a⁃3p levels and the recurrence of infants with proliferative facial hemangioma relapsed after propranolol withdrawal in infants.Methods Ninety⁃three infants with proliferative facial hemangioma were selected,all of whom received propranolol treatment.The recurrence of the hemangioma after treatment was followed up,and the children were divided into a recurrence group(23 patients)and a nonrecurrence group(70 patients).Venous blood was collected before and after treatment,and the se⁃rum levels of miR⁃199a⁃5p and miR⁃378a⁃3p were detected by qRT⁃PCR,and the relationship between the serum levels of miR⁃199a⁃5p and miR⁃378a⁃3p and the recurrence of proliferative facial hemangioma relapsed after propranolol with⁃drawal in infants was analyzed.Results The serum expressions levels of miR⁃199a⁃5p and miR⁃378a⁃3p in non⁃re⁃lapsed group were increased after treatment compared with before treatment(P<0.05),and there was no significant dif⁃ference in the levels of miR⁃199a⁃5p and miR⁃378a⁃3p in the serum of the recurrence group after treatment compared with before treatment(P>0.05).After treatment,the levels of miR⁃199a⁃5p and miR⁃378a⁃3p in the serum of the recur⁃rence group were lower than those of the nonrecurrence group(P<0.05).After treatment,the serum levels of miR⁃199a⁃5p and miR⁃378a⁃3p in patients withⅢ~ⅣNorm grade hemangioma were higher than those in patients withⅠ~ⅡNorm grade hemangioma(P<0.05).Logistic regression analysis showed that the low expression of miR⁃199a⁃5p,low expression of miR⁃378a⁃3p and tumor gradeⅠ~Ⅱafter treatment were risk factors for the recurrence of proliferative facial hemangi⁃oma after propranolol withdrawal in infants(P<0.05).Conclusion Low serum levels of miR⁃199a⁃5p and miR⁃378a⁃3p are associated with the recurrence of proliferative facial hemangioma after propranolol withdrawal in infants.