PTBP1在胶质瘤进展中的作用研究

Role of PTBP1 in glioma progression

目的:探讨多聚嘧啶区结合蛋白1(PTBP1)在胶质瘤进展中的作用。方法:利用多个数据库分析PTBP1在胶质瘤组织和正常脑组织中的表达水平及其与胶质瘤的组织学级别和患者预后的关系。构建稳定敲低PTBP1的LN229细胞系,通过Western印迹和RT-qPCR技术验证细胞中PTBP1的敲低效率。通过EdU实验检测细胞的增殖能力。利用Transwell实验检测细胞的迁移和侵袭能力。结果:PTBP1在胶质瘤组织中较正常脑组织表达更高(P<0.0001),其表达水平随组织学级别的升高而升高(P<0.0001);PTBP1高表达的胶质瘤患者总生存期(OS)明显缩短(P<0.0001);稳定敲低PTBP1的LN229细胞系,细胞的增殖速度变慢(t=3.579,P=0.0373),迁移(t=13.16,P<0.0001)和侵袭能力(t=3.111,P=0.0358)显著下降。结论:PTBP1在胶质瘤中高表达且与胶质瘤的组织学级别和患者不良预后呈正相关;PTBP1促进胶质瘤细胞的增殖、迁移、侵袭。

Objective:To explore the role of polypyrimidine tract-binding protein 1(PTBP1)in glioma progression.Methods:PTBP1 expression level and its relationship with glioma grading and prognosis were analyzed using multiple databases.The glioma cell line LN229 stably silencing PTBP1 was constructed.PTBP1 expression was verified by Western blotting analysis and RT-qPCR.The abilities of cell proliferation,migration and invasion were detected by EdU assay and Transwell assays.Results:The expression of PTBP1 in glioma was higher than that in normal brain tissues(P<0.0001)and increased with glioma grading(P<0.0001).The patients with higher PTBP1 expression exerted a shorter overall survival(OS)(P<0.0001).PTBP1 knockdown inhibited the abilities of cell proliferation(t=3.579,P=0.0373),migration(t=13.16,P<0.0001)and invasion(t=3.111,P=0.0358)significantly.Conclusion:PTBP1 is highly expressed in glioma and is correlated with glioma grading and prognosis.PTBP1 promotes the proliferation,migration and invasion abilities of glioma cells.