贵州主要油茶产区叶枯病病原菌鉴定及室内杀菌剂筛选

Pathogen Identification and Indoor Fungicides Screening of Leaf Blight in Main Camellia Oleifera Oil Producing Areas in Guizhou Province

近年在贵州主要油茶产区发现一种叶枯病病害,为找到叶枯病病原菌,对油茶进行组织分离、培养、纯化,最终鉴定得到叶枯病病原菌拟盘多毛孢菌(Pestalotiopsis kenyana)。对叶枯病病原菌的生理特性进行研究,得到其最适生长温度为20~30℃,最适pH值为6~8。为了进一步评价苯甲·嘧菌酯、肟菌·戊唑醇、苯醚甲环唑、氟环咪鲜胺、氟菌·戊唑醇5种室内杀菌剂对菌丝生长抑制率、菌丝生长速率以及孢子萌发毒力进行测定,结果表明氟环咪鲜胺对菌丝生长毒力的EC_(50)为0.2μg/mL,对孢子萌发毒力的EC_(50)为0.95μg/mL,明显高于其他4种。

In recent years,a leaf blight disease has been discovered in the main Camellia oleifera producing areas in Guizhou.In order to find the pathogen of leaf blight,we have repeatedly isolated,cultured and purified the Camellia oleifera,and finally identified the pathogen of leaf blight as Pestalotiopsis kenyana.The physiological characteristics of the pathogen of the leaf blight disease were studied,and the optimum growth temperature was 20~30℃,and the optimum pH value was 6~8.In order to further evaluate the effects of five indoor fungicides:Benzene·azoxystrobin,Oxime bacteria·tebutazol,Difenoconazole,Fluocyclic imidamine,and Fluomycetes·tebutazolol to mycelia growth inhibition rate,mycelia growth rate and spore germination.The results showed that the EC_(50) of Fluocyclic imidamine to mycelia growth was 0.2μg/mL.The EC_(50) of virulence to spore germination was 0.95μg/mL,which is significantly higher than the other four species.