摘要
目的建立甘肃省肉苁蓉、沙苁蓉、盐生肉苁蓉、兰州肉苁蓉的超高效液相色谱(UPLC)指纹图谱及4种指标性成分含量测定,并进行肉苁蓉药材浸出物含量测定,为肉苁蓉药材的质量评价提供理论依据。方法按照2020年版《中国药典》一部中的醇溶性浸出物下的冷浸法测定浸出物含量;采用色谱柱ACQUITY UPLC®BEH shield RP 18(2.1 mm×100 mm,1.7μm),流动相乙腈-0.1%甲酸水溶液梯度洗脱,体积流量0.1 mL·min^(-1),检测波长330 nm,柱温35℃。结果醇溶性浸出物含量为36%~68%;松果菊苷、肉苁蓉苷A、毛蕊花糖苷、2'-乙酰基毛蕊花糖苷在2μg进样量与峰面积积分值线性关系良好(r≥0.9972),RSD≤3.82%。结论该方法操作简便、精密度高、结果可靠,可用于肉苁蓉、沙苁蓉、盐生肉苁蓉、兰州肉苁蓉的质量评价。
OBJECTIVE To establish UPLC method for the determination of echinacoside,cistanchis glycosides A,verbascoside and 2'-acetyl verbascoside in Herbra Cistanches.METHODS The content of the extract was determined by cold immersion method under the alcohol-soluble extract in the first edition of Chinese Pharmacopoeia 2020.The chromatographic column ACQUITY UPLC BEH SHIELD RP 18(2.1 mm×100 mm,1.7μm)was used.Mobile phase acetonitrile-0.1%formic acid aqueous solution gradient elution;volume flow rate of 0.1 mL·min^(-1).The detection wavelength is 330 nm.The column temperature is maitained at 35℃.RESULTS The content of alcohol soluble extract was 36%~68%.Echinacea glycosides,cistanchis glycosides A,2'-acetyl verbascoside have a good linear relationship(r≥0.9996)with RSD≤3.69%.CONCLUSION The method is simple to operate,high precision and reliable.UPLC can be used for simultaneous determination of four index components in Herbra Cistanches.
作者
陈博
徐小琼
何微微
王莉
吕蓉
朱田田
晋玲
CHEN Bo;Xu Xiao-qiong;HE Wei-wei;WANG Li;LÜRong;ZHU Tian-tian;JIN Ling(College of Pharmacy,Gansu University of Chinese Medicine,Lanzhou 730000,China;Gansu Medical College,Pingliang 744000,China;Northwest Collaborative Innovation Center for Traditional Chinese Medicine,Lanzhou 730000,China)
出处
《中国药学杂志》
CAS
CSCD
北大核心
2021年第19期1563-1570,共8页
Chinese Pharmaceutical Journal
基金
甘肃省教育厅高等学校创新能力提升项目资助(2020A-079)
2018年全国中药资源普查工作项目资助。