TLR3、NF-κB、c-Met蛋白表达与宫颈鳞癌盆腔淋巴结转移的相关性分析

Correlation of TLR3,NF-κB,c-Met protein expression with pelvic lymph node metastasis in patients with cervical squamous cell carcinomas

目的分析Toll样受体(TLRs)、核转录因子-κB(NF-κB)、c-Met蛋白表达与宫颈鳞癌盆腔淋巴结转移的相关性。方法选取2014年3月至2018年1月广州医科大学附属第二医院可行手术的60例Ⅰ、Ⅱ期宫颈鳞癌蜡块标本纳入鳞癌组;选取56例因宫颈高级别鳞状上皮内病变(HSIL)行宫颈锥切术存档蜡块标本纳入HSIL组;选取48例因子宫肌瘤行全子宫切除术存档蜡块标本纳入对照组。采用免疫组化法检测不同宫颈组织中TLR3、NF-κB、c-Met蛋白的表达情况,并进行组间比较。采用单因素χ^(2)检验及Logistic多因素回归分析宫颈鳞癌盆腔淋巴结转移高风险因素。结果各组中均存在TLR3、NF-κB、c-Met蛋白的表达,其中鳞癌组NF-κB、c-Met蛋白阳性表达率显著高于HSIL组和对照组(P<0.05),而鳞癌组TLR3阳性表达率显著低于HSIL组,且HSIL组显著低于对照组(P<0.05);单因素χ^(2)检验及Logistic多因素回归分析结果显示,TLR3、NF-κB、c-Met蛋白的异常表达为宫颈鳞癌盆腔淋巴结转移的高风险因素(P<0.05)。结论宫颈鳞癌组织中存在TLR3、NF-κB、c-Met蛋白的表达异常,其中TLR3的低表达、NF-κB和c-Met蛋白的高表达可能与宫颈癌前病变及宫颈癌的发生发展相关。TLR3、NF-κB、c-Met蛋白的异常表达可能为宫颈鳞癌盆腔淋巴结转移的高风险因素。

Objective To investigate the correlation between the expression of TLR3,NF-κB,and c-Met protein and pelvic lymph node metastasis in patients with cervical squamous cell carcinomas.Methods 60 cases of surgicalⅠ,Ⅱperiod cervical squamous carcinoma group was selected from the Second Affiliated Hospital of Guang-zhou Medical University from March 2014 to January 2018(cervical squamous cell carcinoma group),56 cases of the high level cervical squamous intraepithelial lesion(HSIL)was selected in HSIL group,and 48 cases of undergo total hysterectomy for uterine fibroids normal cervix group was selected in control group.Using immunohistochemical SP method to detect different TLR3 in cervical tissue,the NF-κB,c-Met protein expression,and adopts Sperman rank correlation analysis for different cases characteristics(age,pathological classification,disease stage,lymph node metastasis)and TLR3,NF-κB,c-Met expression correlation analysis,Logistic regression was used to screen the risk factors for pelvic lymph node metastasis of cervical squamous cell carcinoma by univariate screening,and then the risk factors were further analyzed by Logistic multivariate regression.Results The positive expression rate of NF-κB and c-Met protein in cervical squamous cell carcinoma group was significantly higher than that in HSIL group and normal cervical group,and the difference between groups was statistically significant(P<0.05),while the positive expression rate of TLR3 in cervical squamous cell carcinoma group was significantly lower than that in HSIL group,and the difference between groups was statistically significant(P<0.05).Sperman correlation analysis showed that the positive expression of TLR3,NF-κB and c-Met was significantly correlated with pelvic lymph node metastasis(P<0.05),but not with age,pathological grade and disease stage(P>0.05).The results of univari-ate regression analysis showed that TLR3,NF-κB and c-Met were high risk factors for pelvic lymph node metastasis,and the results of further multivariate regression analysis showed that TLR3,NF-κB and c-Met were high risk factors for pelvic lymph node metastasis(OR=0.575,5.154 and 4.210,P<0.05).Conclusions TLR3,NF-κB and c-Met proteins were expressed at abnormal levels in cervical squamous cell carcinoma tissues,TLR3 may inhibit the occurrence and development of cervical squamous cell carcinoma,while NF-κB and c-Met proteins may promote the occurrence and development of cervical squamous cell carcinoma.Abnormal expression of TLR3,NF-κB and c-Met may be the high risk factors for pelvic lymph node metastasis of cervical squamous cell carcinoma.