柠檬酸和枯草芽胞杆菌协同强化万寿菊提取土壤中铀

Synergetic Enhancement of Phytoextraction of Uranium Using Tagetes erecta L From Soil by Citric Acid and Bacillus subtilis

为了高效利用景观植物提取土壤中铀,以芙蓉菊和万寿菊为供试植物,研究了柠檬酸和枯草芽胞杆菌(Bacillus subtilis)对两种植物提取土壤中铀的协同作用。结果表明,添加柠檬酸并加入B.subtilis到土壤可协同促进万寿菊的生长,其生物量和铀的富集总量比单独使用柠檬酸分别提高了48.32%和76.60%,但对芙蓉菊的效果不明显。单独添加柠檬酸、或者同时添加柠檬酸和B.Subtilis,使芙蓉菊和万寿菊叶片中的MDA(malondialdehyde)含量均比对照组显著降低;每种处理方式下的SOD(superoxide dismutase)活性与MDA含量呈正相关;在植物体内铀含量较低时,其POD(peroxidase)活性增高,这表明柠檬酸和B.Subtilis可增加植物的抗氧化性,提高植物的抗铀胁迫能力。

In order to efficiently use landscape plants to extract uranium from soil,Crossostephium chinense(L.)Makino and Tagetes erecta L as the experimental plant,the synergistic effect of citric acid and Bacillus subtilis(B.subtilis)on the phytoextraction of uranium from the soil by the two plants was studied.The experimental results show that adding citric acid and B.subtilis to the soil can synergistically promote the growth of Tagetes erecta L.and its biomass and total uranium enrichment increased by 48.32%and 76.60%,respectively,compared with citric acid alone.But the effect on Crossostephium chinense(L.)Makino was not obvious.Adding citric acid alone or adding citric acid and B.Subtilis at the same time significantly reduced the MDA content in the leaves of Crossostephium chinense(L.)Makino and Tagetes erecta L.compared with the control group.The SOD activity under each treatment was positively correlated with the MDA content.When the uranium concentration is low,the POD activity increases.This indicated citric acid and B.Subtilis could increase antioxidation of Crossostephium chinense(L.)Makino and Tagetes erecta L resistance to oxidation and improve its tolerance to uranium.