灌喂鼠李糖乳杆菌对妊娠后期小鼠粪便菌群多样性影响

Effects of Lactobacillus rhamnoides on the Diversity of Fecal Flora in Late Pregnant Mice

【目的】研究妊娠期小鼠灌喂鼠李糖乳杆菌至分娩前对小鼠粪便中菌群多样性和物种丰度的影响。【方法】选择体重相近(23.33±1.55)g,配种日期相同的昆明妊娠小白鼠30只,将30只妊娠小鼠随机分为2组,每组15只,分别为对照组和试验组。在相同的饲养条件和日粮营养水平下,对照组饲喂基础日粮,试验组饲喂基础日粮的基础上灌喂0.5 mL含有0.125 g鼠李糖乳杆菌的灭菌生理盐水,试验期21 d。【结果】(1)灌喂鼠李糖乳杆菌对小鼠粪便菌群多样性无显著性影响(P>0.05),但降低了试验组Simpson指数。(2)在门水平上,厚壁菌门、拟杆菌门和变形菌门的丰度之和在90%以上,厚壁菌门试验组显著高于对照组(P<0.05),但拟杆菌菌门和变形菌门试验组分别比对照组降低了21.95%(P<0.05)、26.15%(P<0.05)。在科水平上,毛螺菌科、乳酸细菌科和韦荣球菌科试验组显著高于对照组(P<0.05)。在属水平上,乳酸杆菌属、未确定毛螺菌科属与Lachnoclostridium试验组显著高于对照组(P<0.05)。【结论】妊娠期小鼠灌喂鼠李糖乳杆菌,对小鼠粪便菌群多样性无显著性影响,但可以提高小鼠肠道细菌物种丰度,对厚壁菌门、毛螺菌科、乳酸细菌科、韦荣球菌科、乳酸杆菌属、未确定毛螺菌科属和Lachnoclostridium丰度具有显著性影响。

【Objective】The aim of this project is to study the effects oflactobacillus rhamnoides on the diversity and species abundance in feces of mice during pregnancy.【Methods】The test selected 30 Kunming pregnant mice with similar weight(23.33±1.55)g,and with the same mating date and they were randomly divided into 2 groups,15 in each group,respectively,the control group and the test group.Under the same feeding conditions and dietary nutrition level,the control group was fed with the basic diet without any treatment.The mice in the test group were fed with 0.5 mL sterilized saline containing 0.125 g Lactobacillus rhamnoides on the basis of feeding the basic diet,and the test period lasted 21 days.【Results】The results showed that:(1)Lactobacillus rhamnoides had no significant effect on the diversity of fecal flora in mice(P>0.05),but decreased the Simpson index in the experimental group.(2)At the phylum level,the amount of abundance of Firmicutes,Bacteroidetes and Proteobacteria was above 90%,and the Firmicutes was significantly higher than that of the control group(P<0.05),but the Bacteroidetes and Proteobacteria were 21.95%(P<0.05)and 26.15%(P<0.05)lower than those of the control group.At the family level,the test groups of the Lachnospiraceae,Lactobacillaceae and Erysipelotrichaceae were significantly higher than those of the control group(P<0.05).On the genus level,the Lactobacillus,the genus of unidentified_Lachnospiraceae and the Lachnoclostridium test group were significantly higher than those of the control group(P<0.05).【Conclusion】From this,we can see that under the conditions of this experiment,the feeding of Lactobacillus rhamnoides in pregnant mice has no significant effect on the diversity of fecal flora,but it can improve the species abundance of intestinal bacteria in mice,and produces significant effect on the abundance of Firmicutes,Lachnospiraceae,Lactobacillaceae,Erysipelotrichaceae,Lactobacillus,unidentified_Lachnospiraceae and Lachnoclostridium.