基于UPLC-ESI-MS^(n)技术解析清营汤提取物的化学成分

Analysis of the chemical components of Qingying Decoction extract based on UPLC-ESI-MS^(n) technology

目的研究中药复方清营汤的化学成分,为推广其临床应用奠定基础。方法采用Agilent Zorbax SB-C 18(150 mm×3 mm,1.8μm)色谱柱,以1 mL·L^(-1)甲酸-甲醇为流动相,梯度洗脱,采用电喷雾(ESI)离子源,正负离子扫描采集数据,通过超高效液相色谱-电喷雾电离-串联质谱法(UPLC-ESI-MS^(n))解析清营汤提取物的化合物信息。结果建立了清营汤提取物的UPLC色谱图以及UPLC-ESI-MS^(n)总离子流图,根据其质谱裂解信息以及文献对比结果,共得到61个化合物。结论清营汤提取物主要包括黄酮类、环烯醚萜类、苯丙素类、生物碱类、有机酸类和三萜皂苷类等化合物。

Objective To study the chemical components of Qingying Decoction and lay a foundation for its clinical application.Methods An Agilent Zorbax SB-C 18(150 mm×3 mm,1.8μm)column was used for gradient elution with water and methanol of 1 mL·L^(-1) formic acid as mobile phase.The data were collected by electrospray(ESI)ion source and positive and negative ion scanning.The compound information of Qingying Decoction was analyzed by ultra performance liquid chromatography-electron spray Ionization-mass spectrometry(UPLC-ESI-MS^(n)).Results The ultra high performance liquid chromatography(UPLC)chromatogram and UPLC-ESI-MS^(n) total ion flow diagram of the extract of Qingying Decoction were established.According to the information of mass spectrometry and the comparison with literatures,61 compounds were obtained.Conclusion The extracts of Qingying Decoction mainly include flavonoids,iridoids,phenylpropanoids,alkaloids,organic acids,triterpenoid saponins and other compounds.