2株拮抗放线菌复合防治番茄青枯病的研究

Control Effect of Two Antagonistic Actinomycete Mixed Strains against Tomato Bacterial Wilt Disease

为克服单一拮抗菌抑菌活性不够稳定、防治效果差等不足,本文研究了2株优良放线菌菌株之间亲和性及其复合对番茄青枯病的防治效果表明,2株菌之间无明显拮抗作用,将亲和性菌株FX81和FX28等比例同时复合发酵,复合发酵液对番茄青枯病菌的抑菌圈直径达36.0 mm,抑菌效果明显高于单一菌株FX81(27.0 mm)和FX28(28.8 mm)。将2株拮抗菌制成复合菌剂BOF8128对番茄青枯病的盆栽防效为82.03%,比单一菌剂分别提高了8.13%(BOF81)和18.63%(BOF28),同时复合菌剂显著促进了番茄地上部分的生长,干重增加76.58%。由此表明,2株放线菌在防治番茄青枯病方面具有协同增效的作用,有望用于进一步菌肥的开发和应用。

In order to overcome disadvantages of single antagonistic strain in controlling tomato bacterial wilt,such as unstable antibacterial activity or poor control effect, the affinity and disease control efficacy of two selected antagonistic actinomycetes mixed strains were investigated. The results showed that there was no obvious antagonistic effect between the two strains, and the composite fermentation broth of FX81 with FX28 strains resulted in a larger inhibition zone(36 mm) against R. solanacearum growth compared to the single strain FX81(27 mm) and FX28(28.8 mm). The control efficacy of the two mixed strains(BOF8128) on tomato bacterial wilt was 82.03% in potted experiment, which was increased by 8.13%(BOF81) and 18.63%(BOF28) respectively.Meanwhile, the growth of tomato aerial part was significantly promoted, and the dry weight increased by 76.58%.Thus, the two actinomycete strains had significant synergistic effect on the control of tomato bacterial wilt,suggesting that they had the potential to be used in the development and application of microbial fertilizer.