HOTAIR与miR-613表达对食管鳞癌细胞增殖和凋亡影响

EFFECT OF THE EXPRESSION OF HOX TRANSCRIPT ANTISENSE INTERGENIC RNA AND MICRORNA-613 ON THE PROLIFERATION AND APOPTOSIS OF ESOPHAGEAL SQUAMOUS CELL CARCINOMA CELLS

目的探讨HOX转录反义RNA(HOTAIR)和微小RNA(miR)-613在食管鳞癌细胞中的调控关系及其对细胞增殖与细胞凋亡的影响。方法采用实时荧光定量聚合酶链反应(RT-qPCR)检测食管鳞癌细胞中HOTAIR和miR-613的表达情况,双荧光素酶报告基因实验检测HOTAIR和miR-613靶向关系,CCK-8法和流式细胞仪检测细胞增殖和凋亡。结果与其他食管鳞癌细胞相比,人食管鳞癌细胞株Eca-109细胞中HOTAIR表达最高而且miR-613表达水平最低(F=48.56、56.28,P<0.05)。HOTAIR可靶向调控miR-613的表达。下调HOTAIR的表达明显抑制了Eca-109细胞增殖,提高了细胞凋亡和miR-613表达水平(F=205.28~396.53,P<0.05);抑制miR-613表达后,Eca-109细胞增殖能力明显增强,而细胞凋亡能力明显减弱(F=51.04、511.37,P<0.05)。结论下调HOTAIR可通过调控miR-613表达抑制食管鳞癌Eca-109细胞增殖并促进其凋亡。

Objective To investigate the regulatory role of HOX transcript antisense intergenic RNA(HOTAIR) and microRNA-613(miR-613) in esophageal squamous cell carcinoma cells and their effect on cell proliferation and apoptosis. Me-thods Quantitative real-time PCR was used to measure the expression of HOTAIR and miR-613 in esophageal squamous cell carcinoma cells;dual luciferase reporter gene assay was used to detect the targeting relationship between HOTAIR and miR-613;CCK-8 assay and flow cytometry were used to measure cell proliferation and apoptosis. Results Compared with other esophageal squamous cell carcinoma cell lines, the human esophageal squamous cell carcinoma cell line Eca-109 had the highest expression level of HOTAIR and the lowest expression level of miR-613(F=48.56,56.28;P<0.05). HOTAIR showed targeted regulation of miR-613 expression. Downregulation of HOTAIR expression significantly inhibited the proliferation of Eca-109 cells and increased cell apoptosis and the expression level of miR-613(F=205.28-396.53,P<0.05). After the expression of miR-613 was inhibited, Eca-109 cells showed a significant increase in proliferation and a significant reduction in apoptosis(F=51.04,511.37;P<0.05). Conclusion Downregulation of HOTAIR can inhibit the proliferation and promote the apoptosis of Eca-109 cells by regulating the expression of miR-613.