R26-hSCARB2小鼠生理生化指标的测定与分析

Determination of Blood Physiological and Serum Biochemical Values of R26-h SCARB2 Mice

目的测定R26-hSCARB2小鼠的血液生理生化指标,分析性别和周龄对上述指标的影响。方法分别取20只4周龄和8周龄的R26-hSCARB2小鼠,雌雄各半。取静脉血后,测定22项血液生理指标和12项血液生化指标,使用统计学方法分析各指标在性别和周龄间的差异。结果检测的22项生理指标和12项生化指标中,4周龄的R26-hSCARB2小鼠雌雄之间在淋巴细胞绝对值(LYM)、中性粒细胞百分率(NEUT%)及尿素(BUN)指标上具有显著差异(P<0.05),白细胞计数(WBC)、总胆固醇(CHO)及甘油三酯(TG)指标上具有极显著差异(P<0.01)。8周龄的R26-hSCARB2小鼠雌雄之间在WBC、LYM、单核细胞百分率(MON%)、嗜碱性粒细胞百分率(BAS%)、总蛋白(TP)、磷(P)及CHO指标上具有极显著差异(P<0.01);在血小板计数(PLT)、血小板分布宽度(PDW)、中性粒细胞绝对值(NEUT)、嗜酸性粒细胞百分率(EOS%)、白蛋白(ALB)、碱性磷酸酶(ALP)、葡萄糖(GLU)及TG指标上,具有显著差异(P<0.05)。不同周龄的R26-hSCARB2小鼠在WBC、红细胞分布宽度(RDW)、PLT、LYM、单核细胞绝对值(MON)、淋巴细胞百分率(LYM%)、MON%、丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、TP、ALB、ALP、BUN、肌酐(CREA)、葡萄糖(GLU)、钙(Ca)、P、CHO及TG指标上具有极显著差异(P<0.01),在血小板压积(PCT)、平均血小板体积(MPV)、NEUT及NEUT%指标上具有显著差异(P<0.05)。结论性别和周龄都会影响R26-hSCARB2小鼠的血液生理生化指标,但周龄对该小鼠的血液生理生化指标的影响更显著。本实验为R26-hSCARB2小鼠的研究应用提供了基础的数据。

Objective To examine blood physiological and serum biochemical values of R26-h SCARB2 transgenic mice and analyze the influence of genders and ages on these values.Method Four weeks and 8 weeks R26-hSCARB2 mice were taken respectively,with a gender ratio of 1∶110 mice in each group.After venous blood was collected,22 blood physiological values and 12 serum biochemical values were determined,and statistical method were used to analyze the genders and weeks differences of each value.Result There were significant differences(P<0.05)in LYM,NEUT%and BUN,and the extremely significant differences(P<0.01)in WBC,CHO and TG between male and female of 4 weeks R26-h SCARB2 mice.There were significant differences(P<0.05)in EOS%,NEUT,PDW,PLT,ALB,ALP,GLU and TG,and the extremely significant differences(P<0.01)in BAS%,LYM,MON%,WBC,CHO,P and TP between male and female of 8 weeks R26-hSCARB2 mice.There were significant differences(P<0.05)in MPV,NEUT,NEUT%and PCT,and the extremely significant differences(P<0.01)in LYM,LYM%,MON,MON%,PLT,RDW,WBC,ALB,ALP,ALT,AST,BUN,Ca,CHO,CREA,GLU,P,TP and TG between different ages of R26-hSCARB2 mice.Conclusion Both genders and ages can affect the blood physiological and serum biochemical values of R26-hSCARB2 mice,and the effect of ages is greater than that of genders.This study provides data support for the application of R26-h SCARB2 mice.