血管内皮生长因子结合可降解壳聚糖治疗大鼠心肌梗塞的研究

Vascular Endothelial Growth Factor Combined with Degradable Chitosan for the Treatment of Myocardial Infarction in Rats

目的研究血管内皮生长因子(vascular endothelial growth factor,VEGF)结合可降解壳聚糖(degradable chitosan,DC)对心肌梗塞大鼠的治疗作用。方法将32只SD大鼠随机分为假手术组、模型组、VEGF组和VEGF+DC组,每组8只。4组大鼠均采用结扎冠状动脉左前降支的方法进行造模,其中假手术组只穿线不结扎。造模后,向模型组大鼠心肌注射100μL生理盐水,VEGF组大鼠于同一位置注射同体积VEGF溶液,VEGF+DC组大鼠注射VEGF+DC溶液。造模4周后,对所有实验大鼠进行超声心功能检测,考察左心室收缩期及舒张期前壁厚度(LVAW;s和LVAW;d)、左心室收缩期及舒张期内径(LVID;s和LVID;d)、左心室收缩期及舒张期容积(LV Vol;s和LV Vol;d)、左室射血分数(EF%)及短轴缩短率(FS%),血流动力学考察左室舒张末压(EDP)和等容收缩期及舒张期左心室内压力上升最大速率(+dp/dt和-dp/dt),计算左心室指数(LVM/BW)并对心肌进行苏木素-伊红(HE)染色观察各组实验大鼠心肌病理变化。结果 VEGF组和VEGF+DC组大鼠的心肌梗塞均得到有效改善,其中VEGF+DC组的改善作用更加显著。结论 VEGF结合DC对心肌梗塞大鼠的心功能具有改善作用,具有一定的治疗作用。

Objective To study the therapeutic effect of vascular endothelial growth factor( VEGF) combined with degradable chitosan( DC) on myocardial infarction MI rats. Method Thirty two SD rats were randomly divided into the sham operation group,the model group,the VEGF group and the VEGF + DC group,with 8 rats in each group. These rats were modeled by ligating the left anterior descending coronary artery,and the sham operation group was only threaded but not ligated. After modeling,100 μL of normal saline was injected into the myocardium of rats in the control group,rats in VEGF group were injected with the same volume of VEGF solution at the same location,and rats in VEGF + DC group were injected with VEGF + DC solution. After 4 weeks of modeling,all rats were subjected to echocardiographic function testing to investigate the left ventricular systolic and diastolic anterior wall thickness( LVAW;s,LVAW;d),left ventricular systolic and diastolic diameter( LVID;s,LVID);d),left ventricular systolic and diastolic volume( LV Vol;s,LV Vol;d),left ventricular ejection fraction rate( EF%) and short axis shortening rate( FS%),hemodynamic study left ventricular end diastole pressure( EDP)and the maximum rate of increase of left ventricular pressure during isovolumic systole and diastole( + dp/dt,-dp/dt), left ventricular index was calculated and the myocardium was stained with HE to observe the pathological changes of the myocardium in each group of laboratory animals. Result The myocardial infarction of rats in the VEGF group and the VEGF + DC group was effectively improved,and the improvement effect of the VEGF + DC group was more significant. The VEGF combined with DC can significantly improve various cardiac function indexes in rats with myocardial infarction. Conclusion In this study,it can be known that VEGF combined with DC can improve the cardiac function of rats with myocardial infarction and have certain therapeutic effects on myocardial infarction.