冬青油透明蚕体的技术研究

Technical Study on Silkworm Clearing Using Methyl Salicylate

利用生物组织透明技术进行家蚕全形组织展示,对蚕学教学与科研都有特别的意义。该文介绍了一种冬青油(水杨酸甲酯)快速透明蚕体的方法。实验结果表明:冬青油透明蚕体的效果良好,气管丛与神经节显示明显;4种染色方法都能提高蚕体透明的反差,总体效果为次甲基蓝>苏木精>中性红>伊红。其中,中性红和伊红的染色深浅不一、反差相对较差;苏木精染色明显、着色均匀,轮廓显示清晰,但总体反差不明显;次甲基蓝对蚕体不同组织的着色差异较大且容易辨别。

The tissue display of silkworm using tissue clearing technique is of special significance to sericology teaching and relevant scientific research.This paper introduces a technique on silkworm clearing using methyl salicylate.The results have shown that the effect of methyl salicylate on silkworm clearing is good;the four kinds of dyeing could improve the contrast of silkworm tissues,and overall effect follows the order of methylene blue>hematoxylin>toluylene red>eosin.The dyeing of toluylene red and eosin on silkworm tissues are uneven,providing little contrast;the dyeing of hematoxylin is obvious with even coloring,clear display,yet unobvious overall contrast.The dyeing of methylene blue on different tissues of silkworm is heterogeneous and easy to detect.